Project description:Gene expression profiling reveals a potential role of Luteolin in human neuronal stem cells (hNSCs) differentiation . hNSCs purchased from Gibco were treated with 1 μM verbenalin for 24 hours. Microarray gene expression profiling was conducted for biological replicates of hNSCs cultured in differentiation cell culture medium supplemented with Luteolin for 24 hours and untreated control cells cultured in differentiation cell cultured medium .
Project description:Gene expression profiling reveals a potential role of Luteolin in LPS induced depression model LPS depression induced mice were orally treated with luteolin (10 mg/kg body weight) once per day during 8 consecutive days. Microarray gene expression was conducted for isolated mice Neural Stem Cells (NSCs) . Two mice brain were used for each expermient. The Microarray gene expression was conducted on NSCs and hipocampus from LPS depression induced depression mice (LPS group), LPS depression induced depression mice treated with luteolin(LPS+L group), Normal mice (PBS group) and Normal mice treated with luteolin (PBS+L).
Project description:TCQA promoted differentiation of hNSCs at least toward the neuronal lineage and suggests the possibility of using TCQA to promote neurogenesis. When differentiation is induced by growth factor withdrawal, decreased expression of stemness gene and increased expression of NSC fate-promoting genes can be observed. Therefore, we evaluated the effect of TCQA on global gene expression in hNSCs during differentiation at 24h to elucidate the neurogenesis-promoting effects of TCQA.
Project description:Gene expression profiling reveals a potential role of Luteolin in LPS induced depression model LPS depression induced mice were orally treated with luteolin (10 mg/kg body weight) once per day during 8 consecutive days. Microarray gene expression was conducted on mice hippocampal tissues. Two mice brains were used for each expermient . The Microarray gene expression was conducted on hipocampus from LPS depression induced mice model(LPS group), LPS depression induced mice model treated with luteolin(LPS+L group), Normal mice (PBS group) and Normal mice treated with luteolin (PBS+L).
Project description:To investigate the function of Luteolin and siVRK1 on ovarian cancer, we use A2780 and ES2 cell lines treated with siCtrl, siVRK1, DMSO and luteolin to do the experiment. We then performed gene expression profiling analysis using data obtained from RNA-seq .
Project description:To isolate RNA molecules bound to RBM3, we performed CLIP-seq analysis using differentiated hNSCs cultured in neural differentiation medium at 35℃ and 37℃.
Project description:In order to elucidate why mild hypothermia of 35degC could promote neurogenesis of cultured hNSCs, we performed single-cell RNA sequencing (scRNA-seq) to reveal the molecular mechanism.