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Expression data from epidermal human skin (stem cells, transient amplifying cells, differentiated cells)

ABSTRACT: Expression data from epidermal human skin (stem cells, transient amplifying cells, differentiated cells)

PROVIDER: PRJNA623369 | ENA |

REPOSITORIES: ENA

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Expression data from epidermal human skin (stem cells, transient amplifying cells, differentiated cells)

Project description:Performing global gene expression profiling for different types of keratinocytes colonies can provide important information that might help in understanding the biology and ontogeny of epidermal skin stem cells. We used microarrays to detail the global gene expression profiling for different types of keratinocytes colonies including Holoclones (stem cells), Meroclones (transient amplifying cells), and Paraclones (differentiated cells) .

2021-10-20 | GSE148164 | GEO

Single cell expression profiling of human epidermal stem and transit amplifying cells

Project description:To explore which signaling pathways are of importance in maintenance of human epidermal keratinocytes we have established a protocol which allows single cell expression profiling. The protocol was applied to cultured human epidermal keratinocytes, and single cell cDNA libraries from stem and transit amplifying cells were identified based on the expression of known SC markers, MCSP and Dll1. We used microarrays to obtain expression profiles from single cells and identify common denominators that could be important for maintenance and signalling in epidermal stem cells. Keywords: Timecourse

2006-08-04 | GSE4858 | GEO

Single keratinocyte analysis uncovers Foxm1 as a Yap-dependent regulator of human epidermal stem cells (microarray)

Project description:Autologous epidermal cultures can permanently restore a functional epidermis on severely burned patients. Transgenic epidermal grafts do so also in genetic skin diseases as Junctional Epidermolysis Bullosa. Clinical success strictly requires an adequate number of epidermal stem cells, detected as holoclone-forming cells. To date, such cells can be only partially distinguished from the other transient amplifying clonogenic keratinocytes and cannot be prospectively isolated. Here we show that genome-wide single-cell transcriptome analysis performed on primary human epidermal keratinocyte cultures identified categories of genes clearly distinguishing the different clonal types, unveiled that holoclone-forming cells are enriched in genes regulating cell cycle, DNA repair (including telomerase), chromosome segregation and spindle organization, confirmed that human epidermal keratinocytes are hierarchically organized along a continuous, mainly linear trajectory showing that stem cells sequentially generate progenitors producing terminally differentiated cells and uncovered the role of FOXM1 as a YAP-dependent key regulator of normal and adhesion-defective epidermal stem cells.

2021-03-18 | GSE155815 | GEO

Single keratinocyte analysis uncovers Foxm1 as a Yap-dependent regulator of human epidermal stem cells (scRNA-Seq)

2021-03-18 | GSE155816 | GEO

A spatio-temporal characterization of the transcriptional landscape of epidermal development

Project description:The mouse epidermis develops from a single layer of cells at e9.5 to a fully formed organ at birth capable of performing essential functions. The regularity of the epidermal differentiation program creates an excellent system to explore the mechanisms required for this transition from transit amplifying/ stem cell to differentiated cell and to identify key regulators in this biological process. Here we use FACS sorting of transgenic mice to separate the developing epidermis into its differentiating populations. Though many key players in epidermal differentiation have been identified, the challenge of examining the process in vivo has limited discovery of novel associated pathways and proteins. With this profiling we aim to create a map of epidermal development for identificatin of novel regulators of this process.

2017-02-14 | GSE75931 | GEO

Smad3 binding regions in differentiated human progenitor epidermal keratinocytes (HPEK).

Project description:Smad3 binding regions in differentiated human progenitor epidermal keratinocytes (HPEK).

| PRJNA747268 | ENA

Transcriptional profiles of murine E13 epidermal cells and P4 epidermal basal cells

Project description:Transcriptional profiles of murine E13 epidermal cells and P4 epidermal basal cells

| PRJNA494644 | ENA

Dedifferentiation of Gata6+ epidermal cells

Project description:Dedifferentiation of Gata6+ epidermal cells

| PRJNA731921 | ENA

Both wnt signaling and epidermal stem cell-derived exosomes are involved in epidermal development

Project description:Millions suffer from skin diseases. Functional epidermal stem cells are needed in skin therapy or drug screening in vitro. We obtained functional epidermal stem cells with intact stemness and cell junctions by treating them with wnt3a. Moreover, epidermal stem cell-derived exosomes were useful in epidermal development. Finally, functional epidermal 3D organoids with polarity were cultured using wnt3a and the supernatant derived from epidermal stem cells and fresh medium in a 1:1 ratio. These results provide novel directions for the improvement of skin organoids and their potential in clinical application.

2020-07-14 | PXD020351 |

Transcriptional profiles of murine E13 epidermal cells and P4 epidermal basal cells

Project description:Transcriptional profiling of mouse epidermal cells at embryonic day 13 (E13) compared with epidermal basal cells at postnatal day 4 (P4), both isolated from Krt14-H2BGFP mouse skin. Goal was to identify the genes differentially expresssed in E13 epidermal cells vs P4 epidermal basal cells.

2018-10-05 | GSE120824 | GEO

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