Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Culturing of Daoy cells in astrocyte conditioned media

ABSTRACT: Culturing of Daoy cells in astrocyte conditioned media

PROVIDER: PRJNA625003 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Culturing of Daoy cells in astrocyte conditioned media

Project description:Astrocytes are important cells within the medulloblastoma microenvironment. Therefore, we assessed how astrocyte secreted factors may alter Daoy (human MB cell line; ATCC HTB-186) cell gene expression.

2020-04-14 | GSE148540 | GEO

Wildtype and Gde3 knockout conditioned media from primary astrocyte culture

Project description:Primary astrocyte cultures were prepared from wildtype and Gde3 knockout mouse brain. Conditioned media was collected, concentrated and analyzed using TMT-MS.

2023-03-20 | MSV000091515 | MassIVE

Astrocyte conditioned media-dependent Phosphatidylinositol 3-kinase interactome in mouse hippocampal neuron cell line Ht22

Project description:Astrocytes can support neuronal survival through a range of secreted signals that protect against neurotoxicity, oxidative stress, and apoptotic cascades. To identify proteins contributing to protective intracellular neuronal signalling originating from astrocytes, endogenous PI3K was immunoprecipitated from Ht22 cells exposed to primary astrocyte conditioned media (ACM) or cell free media (CFM), followed by iTRAQ-based quantitative proteomic analysis.

2021-09-09 | PXD022200 | Pride

Mouse ES-derived motor neurons (ES-MN) exposed to SOD1 mutant mouse astrocyte conditioned media

Project description:This datased was used to obtain a genome-wide expression signature for the early response of mouse motor neurons to mutant SOD1 astrocytes conditioned media. Neurons, far from living in isolation, are surrounded by a host of other neuronal and non-neuronal cells, such as astrocytes. The latter entertain complex functional interactions with neighboring neurons, which, under normal conditions, are important for the their well-being. In pathological situations, however, altered astrocyte behavior may contribute to the demise of neighboring neurons. Such non-cell autonomous pathogenic scenario is increasingly considered in a variety of disorders, including amyotrophic lateral sclerosis (ALS), the most frequent adult-onset paralytic disorder. Assembly and interrogation of gene regulatory models has helped elucidate causal mechanisms responsible for the presentation of several tumor-related phenotypes. To systematically elucidate the effectors of neurodegeneration in a model of ALS, we first developed techniques for the efficient purification of motor neurons (MNs), the primary target of ALS neurodegenerative process. We then generated gene expression profiles to fully characterize the critical timepoints associated with initiation and commitment of MN degenerative progression in an in vitro murine mutant SOD1 (mSOD1) model of ALS. ES cells were derived from transgenic Hlxb9-GFP1Tmj mice expressing eGFP and CD2 driven by the mouse HB9 promoter. These cells were then differentiated into motor neurons (ES-MN) as described previously [PMID 12176325] ES-MN were exposed to non-transgenic (NTg), G93A mutant SOD1 (mSOD1) or wtSOD1 over-expression astrocytes conditioned media for 0 days (time zero control), 1 day, and 3 days. Total RNA was extracted and profiled by RNAseq.

2014-12-17 | E-GEOD-49023 | biostudies-arrayexpress

Effect of conditioned media from Aeromonas caviae on the transcriptomic changes of the porcine isolates of Pasteurella multocida

Project description:To investigate the interaction between two porcine isolates of P. multocida (PM2 for type D and PM7 for type A) with Aeromonas caviae by co-culturing P. multocida in the conditioned media prepared from A. caviae

2022-12-01 | GSE211697 | GEO

NRVM Conditioned Media Proteomics

Project description:Proteomic results from NRVM conditioned media overexpressing GRK2

2024-04-22 | PXD050356 | Pride

RNA-Sequencing of Astrocytes Stimulated with Activated Endothelial conditioned media (ECM) and Activated Microglia conditioned media (MCM)

Project description:The goal of this study is to compare the mRNA transcriptome of astrocytes treated with Activated Microglia and Endothelial conditioned media

2022-03-18 | GSE175485 | GEO

Mouse ES-derived motor neurons (ES-MN) exposed to SOD1 mutant mouse astrocyte conditioned media

2014-12-17 | GSE49023 | GEO

Human osteoblast treated with chemotherapy-treated bone marrow stromal cell conditioned media

Project description:Global expression profile of human osteoblast treated with chemotherapy-treated bone marrow stromal cell conditioned media, compared to human osteoblast cells treated with diluent-control bone marrow stromal cell conditioned media. Goal is to identify genes regulated by chemotherapy in osteoblasts.

2010-09-30 | GSE17842 | GEO

RNA-Seq comparison of pancreatic stellate cells (PSCs) cultured with control media, TGF-beta, pancreatic tumor organoid conditioned media or pancreatic tumor organoid conditioned media in the presence of ERBBi (neratinib)

Project description:We investigated the role of ERBB signaling in PSCs cultured with TGF-beta (myCAFs) or pancreatic tumor organoid conditioned media (with or without ERBBi) compared to PSCs cultured with control media (quiescent PSCs)

2023-11-30 | GSE219180 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data