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Cell-intrinsic differences between human tracheal epithelial cells from children and adults [FACS-sorted basal cells]

ABSTRACT: Cell-intrinsic differences between human tracheal epithelial cells from children and adults [FACS-sorted basal cells]

PROVIDER: PRJNA625888 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11560061_1.fastq.gz	Fastqsanger.gz
	SRR11560061_2.fastq.gz	Fastqsanger.gz
	SRR11560062_1.fastq.gz	Fastqsanger.gz
	SRR11560062_2.fastq.gz	Fastqsanger.gz
	SRR11560063_1.fastq.gz	Fastqsanger.gz

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Cell-intrinsic differences between human tracheal epithelial cells from children and adults [FACS-sorted basal cells]

Project description:The airway epithelium is a key protective barrier whose integrity is preserved by the self-renewal and differentiation of basal progenitor cells. Epithelial cells are central to the pathogenesis of multiple chronic lung diseases for which age is a principle risk factor. Children are also less susceptible to SARS-CoV-2 infection, suffer less severe symptoms than adults and have a lower rate of mortality. Few studies have addressed differences between airway epithelial cells in children and adults. Here, we perform bulk RNA sequencing studies in laser-captured whole epithelium, FACS-sorted basal cells and cultured basal cells, as well as in vitro cell proliferation experiments, to address the intrinsic molecular differences between paediatric and adult airway basal cells. We find that, while the cellular composition of the paediatric and adult tracheal epithelium is broadly similar, in cell culture, paediatric airway epithelial cells displayed higher colony forming ability, better in vitro growth and outcompeted adult cells in competitive proliferation assays. Although recurring differences between airway epithelial gene expression were seen between samples from children and adults, RNA sequencing showed broad conservation of transcriptional programmes. Genes associated with SARS-CoV-2 infection were not differentially expressed between children and adults, although individuals showed some variability in their expression of viral infection-associated genes. Our results chart important cell intrinsic differences in transcriptional profile and regenerative capacity between tracheal epithelial cells of children and adults.

2020-04-20 | GSE148816 | GEO

Cell-intrinsic differences between human tracheal epithelial cells from children and adults [cultured basal cells]

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Cell-intrinsic differences between human tracheal epithelial cells from children and adults [laser-captured fresh epithelial cells]

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Cell-intrinsic differences between human tracheal epithelial cells from children and adults [cultured basal cells]

Project description:Cell-intrinsic differences between human tracheal epithelial cells from children and adults [cultured basal cells]

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FACS sorted podocytes, mesangial cells

Project description:FACS sorted glomerular kidney cells from a single mouse were subjected to proteomics.

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Cell-intrinsic differences between human tracheal epithelial cells from children and adults

Project description:Cell-intrinsic differences between human tracheal epithelial cells from children and adults

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Cell-intrinsic differences between human tracheal epithelial cells from children and adults

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-04-20 | GSE148818 | GEO

Cell-intrinsic differences between human tracheal epithelial cells from children and adults [laser-captured fresh epithelial cells]

Project description:Cell-intrinsic differences between human tracheal epithelial cells from children and adults [laser-captured fresh epithelial cells]

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Proteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations

Project description:The metabolic status of individual cells in microbial cultures can differ being relevant for biotechnology, environmental and medical microbiology. However, it is hardly understood in molecular detail due to limitations of current analytical tools. Here, we demonstrate that FACS in combination with proteomics can be used to sort and analyze cell populations based on their metabolic state. A previously established GFP reporter system was used to detect and sort single Corynebacterium glutamicum cells based on the concentration of branched chain amino acids (BCAA) using FACS. A proteomics workflow optimized for small cell numbers was used to quantitatively compare proteomes of a ?aceE mutant, lacking functional pyruvate dehydrogenase (PD), and the wild type. About 800 proteins could be quantified from 1,000,000 cells. In the ?aceE mutant BCAA production was coordinated with upregulation of the glyoxylate cycle and TCA cycle to counter the lack of acetyl CoA resulting from the deletion of aceE.

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Mouse tracheal pseudostratified vs hillock basal stem cells

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