Project description:Single cell RNA-seq analysis for spleen tissue isolated from mice under steady state, and subjected to phenohydrazine (PHZ) and bleeding treatment respectively. Single cell RNA-seq analysis for bone marrow cells isolated from mice under steady state.
Project description:More insights into the character differences between ChAT+ and ChAT- NK cells were obtained based on the gene-expression patterns. A clear delineation between ChAT+ NK cells and ChAT- NK cells was observed, with a total of 300 genes over-expressed and 941 genes under-expressed significantly in the ChAT+ subset. It will provide evidences for further investigation into their functional characters
Project description:Biological triplicates of the 4 stages of eosinophil maturation (from I to IV) were sorted via FACS from the bone marrow of mice in the steady state and in IL-33 induced eosinophilia, allowing to study the transcriptional changes occurring during eosinophil maturation in both conditions.
Project description:Circulating monocytes are crucial for tissue resident macrophage (TRM) replenishment. While a temporary loss of TRMs does not impose immediate danger to the host in sterile conditions, the niche comprising of dying macrophages in the presence of pathogenic bacteria predisposes the host to increased morbidity if these cells are not effectively replaced. Importantly, monocytes have been shown to be susceptible to cell death after executing effector functions such as phagocytosis. Hence, the demands and contribution of monocytes in pathogen clearance and macrophage differentiation is perplexing. Interestingly, we show that proliferating transitional pre-monocytes (TpMos), an immediate precursor of mature Ly6Chi monocytes (MatMos), were mobilized into the periphery in response to acute bacterial infection and sepsis. Since TpMos are absent in the circulation in the steady state, we aim to understand how TpMo recruitment into the periphery may result in differing functions from MatMos during sepsis.