Characterization of metabolic patterns in mouse oocytes during meiotic maturation
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA627216 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR11583822_1.fastq.gz | Fastqsanger.gz | |||
| SRR11583822_2.fastq.gz | Fastqsanger.gz | |||
| SRR11583823_1.fastq.gz | Fastqsanger.gz | |||
| SRR11583823_2.fastq.gz | Fastqsanger.gz | |||
| SRR11583824_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 12 |
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Project description:Well balanced and timed energy metabolism is essential for making a high quality egg. However, the metabolic framework that supports oocyte development remains poorly understood. Here we obtained the temporal metabolome profiles of mouse oocytes during in vivo maturation by isolating large number of cells at key stages. In parallel, quantitative proteomic analyses were conducted to bolster the metabolomic data, synergistically depicting the global metabolic patterns in oocytes. In particular, we discovered novel metabolic features during oocyte maturation, such as the fall in polyunsaturated fatty acids (PUFAs) level and the active serine-glycine-one-carbon (SGOC) pathway. Using functional approaches, we further identified two key targets (NKAP and BTG4) through which arachidonic acid (ARA) inhibits meiotic maturation, and demonstrated the control of epigenetic marks in maturing oocytes by SGOC network. Our data serves as a broad resource on the dynamics occurring in metabolome and proteome during oocyte maturation, and provides opportunities for predicting and improving oocyte quality.
2020-12-21 | GSE149053 | GEO
Project description:Well-balanced and timed metabolism is the prerequisite for optimal oocyte development. To date, numerous studies have focused on the utilization of exogenous substrates by oocytes, whereas the underlying mechanism of intrinsic regulation during meiotic maturation is less characterized. Herein, we performed an integrated analysis of parallel metabolomics and transcriptomics by isolating porcine oocytes at three time points, cooperatively depicting the global picture of the metabolic patterns during oocyte maturation. In particular, we identified the metabolic features of porcine oocytes during meiotic maturation, such as the fall in bile acids, the active one-carbon metabolism and a progressive decline in nucleotide metabolism. Collectively, the current study not only provide a comprehensive multiple omics data resource, but also may promote the discovery of biomarkers in the prediction and improvement of oocyte quality.
2023-01-25 | GSE222399 | GEO
Project description:Spermidine promotes meiotic maturation of porcine oocytes
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Project description:Poly(A) polymerase α (PAPα), as the specific mRNA polyadenylation enzyme in the cytoplasm of mammalian oocytes, is essential for oocytes to exclude the first polar body. However, PAPα knockout did not affect germinal vesicles breakdown (GVBD) of oocytes, and the mechanism needs to be further explored. In this study, we identified that PAPα work together with poly(A)-bound RNA binding protein PABPN1 to promote the rupture of germinal vesicles in mammalian oocytes. The protein level of Pabpn1 gradually increases with the meiotic maturation of oocytes. The oocytes specifically knocked out Pabpn1 at the primary follicle stage could develop into the fully grown (FGO) stage, but hardly could enter into the meiotic process. The activated form of CDK1 was injected into Pabpn1-null oocytes, the oocytes could enter into meiotic process. The translational activity of Pabpn1-null oocytes was significantly lower than that of wild-type oocytes during meiosis. In particular, the expression level of protein (BTG4 and CDC25), which were essential for the meiotic maturation of oocytes, were significantly decreased. Therefore, during the oocyte meiosis process, PABPN1 and PAPα jointly promote the oocyte to enter the meiosis process.
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Project description:Spermidine is involved in a variety of biological processes, including DNA metabolism, autophagy and aging. Previous studies have shown that spermidine can increase the percentage of mouse oocytes developing into blastocysts after in vitro fertilization. However, none of the past studies elucidated the effects of spermidine supplementation on porcine oocyte maturation. Here, we choose appropriate dose of spermidine to be added to the maturation medium during in vitro maturation (IVM) to verify whether spermidine can actively promote the maturation of porcine oocytes. Our study provided substantial evidence that spermidine exposure promoted the porcine oocyte meiotic maturation. In addition, single-cell transcriptome analysis identified the target effectors of spermidine actions in porcine oocytes, further demonstrating that spermidine exposure enhanced mitochondrial distribution and function, leading to a reduced excessive oxidative stress-induced DNA damage and early apoptosis of porcine oocytes. These findings demonstrate that spermidine not only delays ageing and cancer treatment, but also improves the quality of germ cells, which may be less likely to suffer from sterility or infertility in humans and animals.
2024-11-30 | GSE217447 | GEO
Project description:Well-balanced and timed metabolism is essential for making a high-quality egg. However, the metabolic framework that supports oocyte development remains poorly understood. Here, we obtained the temporal metabolome profiles of mouse oocytes during in vivo maturation by isolating large number of cells at key stages. In parallel, quantitative proteomic analyses were conducted to bolster the metabolomic data, synergistically depicting the global metabolic patterns in oocytes. In particular, we discovered the metabolic features during meiotic maturation, such as the fall in polyunsaturated fatty acids (PUFAs) level and the active serine-glycine-one-carbon (SGOC) pathway. Using functional approaches, we further identified the key targets mediating the action of PUFA arachidonic acid (ARA) on meiotic maturation and demonstrated the control of epigenetic marks in maturing oocytes by SGOC network. Our data serve as a broad resource on the dynamics occurring in metabolome and proteome during oocyte maturation.
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Project description:PABPN1 promotes meiotic maturation of oocytes by interacting with PAPOLA
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Project description:The global phosphorylation landscape of mouse oocytes during meiotic maturation
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Project description:Fully grown oocytes remain transcriptionally quiescent, yet many maternal mRNAs are synthesized and retained in growing oocytes. We now know that maternal mRNAs are stored in a structure called the mitochondria associated ribonucleoprotein domain (MARDO). But the components and functions of MARDO remain elusive. Here, we found that LSM14B knockout prevents the proper storage and timely clearance of mRNAs (including Cyclin B1, Btg4, and other mRNAs that are translationally activated during meiotic maturation), specifically by disrupting MARDO assembly during oocyte growth and meiotic maturation. With decreased levels of storage and clearance, the LSM14B knockout oocytes failed to enter meiosis II, ultimately resulting in female infertility. Our results demonstrate the function of LSM14B in MARDO assembly, couple the MARDO with mRNA clearance and oocyte meiotic maturation
2023-10-17 | PXD042761 | Pride
Project description:Oocyte maturation refers to oocytes at the germinal vesicle stage progressing into metaphase II (MII) stage of development. Even though numerous studies have shown key genes and potential important signalling cascades, which drive the GV to MII transition, a system-wide analysis of underlying differences at gene level and especially at transcript level between the two developmental stages of the oocyte is still lacking. For this, we profiled and analysed RNA from pig oocytes across meiotic maturation (GV, MII and damaged, n=15). We detected 22,516 genes for each sample across meiotic maturation. Principal Component analysis of the data clustered the samples in three stages of development (GV, MII and damaged). Differential expression of genes between the three stages will then be used to delineate the pathways which are up-/down-regulated during these developmental stages. Besides, differential transcript usage will be used to identify the difference of oocytes at distinct developmental stages at isoform level, which might be ignored by traditional differential gene expression analysis.
2024-07-11 | GSE237026 | GEO