Project description:The gene expression during early (30min) and late (2.5hrs) infection of Mycobacteriophage Giles was analyzed, as well as in a Giles lysogen. Total RNA was isolated, depleted of rRNA and prepped for sequencing (Illumina) during early and late infection of Giles and a lysogen.
Project description:The goal of this sequencing project was to measure transcription levels of ZoeJ mycobacteriophage during different stages of the lifecycle, including lysogeny.
Project description:The gene expression during early (30min) and late (2.5hrs) infection of Mycobacteriophage Giles was analyzed, as well as in a Giles lysogen.
Project description:The gene expression during early (30min) and late (210 minutes) infection of Mycobacteriophage Adephagia was analyzed, as well as in a Adephagia lysogen.
Project description:Provided herein is the first evidence that mycobacteriophage, indeed phage of any kind, have surface proteins (capsid and/or tail tube proteins) that are O-glycosylated. The glycosyltranferases that glycosylate these proteins are encoded in the phage genomes. In addition, for the three mycobacteriophages invested in this study, the glycans appear to be attached exclusively at a C-terminal serine residue. Each phage expresses a unique and novel O-glycan. The LC-MS files deposited here are the preliminary evidence that these mycobacteriophage proteins are glycosylated. A follow-up investigation will examine the composition and structure of the O-glycans in more detail.