Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exosomal microRNAs secreted by induced pluripotent stem cell-derived cardiac cells

ABSTRACT: Exosomal microRNAs secreted by induced pluripotent stem cell-derived cardiac cells

PROVIDER: PRJNA627904 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11605206.fastq.gz	Fastqsanger.gz
	SRR11605207.fastq.gz	Fastqsanger.gz
	SRR11605208.fastq.gz	Fastqsanger.gz
	SRR11605209.fastq.gz	Fastqsanger.gz
	SRR11605210.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Exosomal microRNAs secreted by induced pluripotent stem cell-derived cardiac cells

Project description:We report the secreted microRNAs from extracellular vesicles released from (i) cardiomyocytes, (ii) cardiac fibroblasts, (iii) endothelial cells differentiated from identical lines of normal human induced pluripotent stem cells and (iv) undifferentiated induced pluripotent stem cells.

2020-07-01 | GSE149290 | GEO

Profile analysis of endogenous and exosomal microRNAs in human colon cancer cell lines-1

Project description:MicroRNAs (miRNAs) are intrinsic regulators in the various cellular processes, and their abnormalities are considered to be involved in the onset of human disorders, including cancer. Circulating miRNA is focused as new cancer biomarker however it is regarded that circulating RNA are released not only from tumor but also by various pathways. Recently, exosomes, small membrane vesicles, have been a major interest in cancer research field, because of their unique biological properties. Exosomes are secreted from various cells and the components (Lipids, mRNAs, miRNAs and proteins) reflect origin of the cells secreting them. Identification of exosomal miRNAs from cancer cells is expected to provide useful biomarkers of cancer. To identify specific exosomal miRNAs as candidate biomarkers for colorectal cancer, we compared exosomal miRNA profiles of 5 colon cancer cell lines with that of normal colon-derived epithelial cells, and isolated a subset of miRNAs as commonly-secreted miRNAs from colon cancer cells Endogenously expression of microRNAs were analyzed by Agilent Human miRNA V3 Microarray (G4470C) using total RNAs of human colon-derived FHC cells and human colon cancer cell lines (HCT116 cells and SW480 cells) at two independent experiments. Exosomal microRNAs were analyzed by microRNA microarray using total RNAs of exosomes from conditioned media of FHC cells, HCT116 cells, and SW480 cells at three independent experiments.As negative control of exosomal microRNAs in conditioned media, FBS-exosomal microRNAs were analyzed at four independent experiments. Exosomes were prepared by step-wise ultra-centrifugation methods. RNA was prepared by Trizol or Trizol-LS reagent (Invitrogen) and RNeasy mini spin column (Qiagen).

2014-04-01 | E-GEOD-39814 | biostudies-arrayexpress

microRNAs involved in cardiac differentiation from human pluripotent stem cells

Project description:microRNAs are post transcriptional regulators involved in several biological processes. The main objective of this project is to identify all the microRNAs involved in the regulation of the cardiac differentiation from human pluripotent stem cells. The final goal is to understand RNA networks related to cardiac lineage commitment during human embryonic development.

2018-04-06 | GSE108021 | GEO

Profile analysis of endogenous and exosomal microRNAs in human colon cancer cell lines-2

Project description:MicroRNAs (miRNAs) are intrinsic regulators in the various cellular processes, and their abnormalities are considered to be involved in the onset of human disorders, including cancer. Circulating miRNA is focused as new cancer biomarker however it is regarded that circulating RNA are released not only from tumor but also by various pathways. Recently, exosomes, small membrane vesicles, have been a major interest in cancer research field, because of their unique biological properties. Exosomes are secreted from various cells and the components (Lipids, mRNAs, miRNAs and proteins) reflect origin of the cells secreting them. Identification of exosomal miRNAs from cancer cells is expected to provide useful biomarkers of cancer. To identify specific exosomal miRNAs as candidate biomarkers for colorectal cancer, we compared exosomal miRNA profiles of 5 colon cancer cell lines with that of normal colon-derived epithelial cells, and isolated a subset of miRNAs as commonly-secreted miRNAs from colon cancer cells Endogenously expression of microRNAs were analyzed by Agilent Human miRNA V3 Microarray (G4470C) using total RNA of three human colon cancer cell lines (HT-29 cells, SW48 cells, and RKO cells) at two independent experiments. Exosomal microRNAs were analyzed by microRNA microarray using total RNA of exosomes from conditioned media of three human colon cancer cell lines, HT-29 cells, SW48 cells, and RKO cells at three independent experiments. Exosomes were prepared by step-wise ultra-centrifugation methods. RNA was prepared by Trizol or Trizol-LS reagent (Invitrogen) and RNeasy mini spin column (Qiagen).

2014-04-01 | E-GEOD-39832 | biostudies-arrayexpress

microRNAs involved in cardiac differentiation from human pluripotent stem cells

Project description:microRNAs involved in cardiac differentiation from human pluripotent stem cells

| PRJNA422248 | ENA

Profile analysis of endogenous and exosomal microRNAs in human colon cancer cell lines-2

2014-04-01 | GSE39832 | GEO

Profile analysis of endogenous and exosomal microRNAs in human colon cancer cell lines-1

2014-04-01 | GSE39814 | GEO

The profile of miRNAs secreted into the supernatant during cardiac differentiation and maturation from human induced pluripotent stem cells verified by miRNA array analysis.

Project description:We performed miRNA array analysis to analyze the miRNAs secreted into the supernatant during the mesoderm/cardiac differentiation and maturation process from human induced pluripotent stem cells (hiPSCs). Supernatant samples were collected on day -1, day 3, day 5, day 7, day 9, day 21, day 35, and day 51.

2023-09-22 | GSE240790 | GEO

Circulating exosomal microRNAs contribute to the antimigraine effects of acupuncture

Project description:We conducted this study to determine whether exosome regulation underlies the antimigraine mechanisms of acupuncture. By comparing serum samples from patients with migraine and healthy controls using high-throughput small RNA sequencing technology , we identified 705 exosomal microRNAs that are differentially expressed in patients with migraine, and this set of 705 microRNAs included five that are particularly well characterised (hsa-miR-369-5p, hsa-miR-1268b, hsa-miR-145-5p, hsa-miR-222-5p, and hsa-miR-4488). By comparing serum samples collected from patients with migraine before and after acupuncture treatment, we showed that acupuncture normalised the expression levels of those five well-characterised exosomal microRNAs.

2024-02-12 | GSE198274 | GEO

Circulating exosomal microRNAs as predictive biomarkers of neoadjuvant chemotherapy response in breast cancer

Project description:Circulating exosomal microRNAs as predictive biomarkers of neoadjuvant chemotherapy response in breast cancer

2021-12-31 | GSE182951 | GEO