Project description:Periodic fever is the most characteristic clinical feature of human malaria. However, how parasites survive malarial febrile episodes, which often involve temperatures of >40ºC, is not known. Plasmodium falciparum cultures adapted to periodic heat shock were used to identify PfAP2-HS as a transcription factor that is essential for survival at febrile temperatures. Transcriptomic analysis was performed to compare the effect of heat shock in parasites presenting the wild type form of PfAP2-HS (10E) and parasites that have defects in this protein, either a premature stop codon mutation (10G) or the deletion of the entire pfap2-hs gene (10E_pfap2-hs). A timecourse analysis including samples taken before, during and after heat shock revealed that the initial phase of the PfAP2-HS-dependent response is largely restricted to hsp70-1 and hsp90.
Project description:Periodic fever is the most characteristic clinical feature of human malaria. However, how parasites survive malarial febrile episodes, which often involve temperatures of >40ºC, is not known. To understand the molecular basis of heat shock (HS) resistance in Plasmodium falciparum, we took advantage of previously developed P. falciparum lines adapted to periodic HS (3D7-A-HS) and their non-selected controls maintained in parallel (3D7-A). Their ability to adapt in only a few cycles suggested that the parental parasite population contained a selectable subset of parasites resistant to HS. Transcriptomic analysis of both 3D7-A-HS and 3D7-A was used to study the differences at a transcriptional level that could be related with the HS-resistant phenotype.
Project description:This SuperSeries is composed of the following subset Series: GSE25878: Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription (expression) GSE25879: Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription (CGH) Refer to individual Series
Project description:Transcriptomic Analysis of Cultured Sporozoites of P. falciparum RNA-seq reads from each of three developmental stages (2 replicates per sample) were mapped to the reference Plasmodium falciparum genome, and gene expression levels were calculated for each sample.