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LncRNA sequencing in dorsal root ganglion cultured with high sugar content

ABSTRACT: LncRNA sequencing in dorsal root ganglion cultured with high sugar content

PROVIDER: PRJNA628904 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11625081_1.fastq.gz	Fastqsanger.gz
	SRR11625081_2.fastq.gz	Fastqsanger.gz
	SRR11625082_1.fastq.gz	Fastqsanger.gz
	SRR11625082_2.fastq.gz	Fastqsanger.gz
	SRR11625083_1.fastq.gz	Fastqsanger.gz

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LncRNA sequencing in dorsal root ganglion cultured with high sugar content

Project description:Primary isolated rat dorsal root ganglion nerve cells (DRGs) were cultured with high glucose in vitro and divided into normal culture group and high glucose culture group

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Neuronal cell lines as model dorsal root ganglion neurons: a transcriptomic comparison

Project description:Aim: Dorsal root ganglion neuron-derived immortal cell lines including ND7/23 and F-11 cells have been used extensively as in vitro model systems of native peripheral sensory neurons. However, while it is clear that some sensory neuron-specific receptors and ion channels are present in these cell lines, a systematic comparison of the molecular targets expressed by these cell lines with intact peripheral neurons is lacking. Methods: we examined the expression of RNA transcripts in the human neuroblastoma-derived cell line, SH-SY5Y, and two dorsal root ganglion hybridoma cell lines, F-11 and ND7/23, using Illumina next-generation sequencing. Results: The expression profile of these three cell lines did not resemble any specific dorsal root ganglion neuron subclass. The cell lines lacked many markers for nociceptive sensory neurons, such as the transient receptor potential V1 gene, but expressed markers for both myelinated and unmyelinated neurons. Conclusion: This paper provides insights into the receptor repertoire expressed in common dorsal root ganglion neuron-derived cell lines, and illustrates the limits and potentials of these cell lines as tools for neuropharmacological exploration.

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RNA-sequencing of lidocaine treatment on rat dorsal root ganglion neurons

Project description:We conducted RNA-sequencing of lidocaine hydrochloride in treating rat dorsal root ganglion neurons to detect lidocaine’s effect of transcriptome profiling changes compared with control.

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RNA-sequencing of injured cultured dorsal root ganglion neurons over time

Project description:RNA-sequencing of injured cultured dorsal root ganglion neurons over time

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MicroRNA profiling in dorsal root ganglion after sciatic nerve injury

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