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Identification of genome-wide binding sites of Psi, Myc and Pol and gene expression following Psi or Myc knockdown in Drosophila larval wing tissue

ABSTRACT: Identification of genome-wide binding sites of Psi, Myc and Pol and gene expression following Psi or Myc knockdown in Drosophila larval wing tissue

PROVIDER: PRJNA629796 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11680550.fastq.gz	Fastqsanger.gz
	SRR11680551.fastq.gz	Fastqsanger.gz
	SRR11680552.fastq.gz	Fastqsanger.gz
	SRR11680553.fastq.gz	Fastqsanger.gz
	SRR11680554.fastq.gz	Fastqsanger.gz

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RNA-sequencing following Psi or Myc knockdown in Drosophila larval wing tissue

Project description:RNA-seq analysis of gene expression changes following the depletion of P-element somatic inhibitor (Psi) or Myc via RNAi in wing discs of mid-third instar larvae, compared with control. Differential splicing analysis was also performed on the Psi RNAi dataset.

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RNA-sequencing following Psi or Myc knockdown in Drosophila larval wing tissue

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Identification of genome-wide binding sites of Psi, Myc and Pol in Drosophila imaginal wing discs using Targeted DamID

Project description:Identification of genome-wide binding sites of Psi, Myc and Pol in Drosophila imaginal wing discs using Targeted DamID

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Identification of genome-wide binding sites of Psi, Myc and Pol and gene expression following Psi or Myc knockdown in Drosophila larval wing tissue

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:The molecular mechanisms regulating tissue size represent an unsolved puzzle in developmental biology. One signaling pathway controlling growth of the Drosophila wing is Dpp. Dpp promotes growth via repression of the transcription factor Brinker. The transcriptional targets of Brinker that control cell growth and proliferation, however, are not yet fully elucidated. We report here a genome-wide ChIP-seq of endogenous Brinker from wing imaginal discs. We identify the growth regulator Myc as a target of Brinker and show that Myc together with the microRNA bantam explain a large fraction of the growth inhibition caused by Brinker. This work sheds light on the effector mechanisms by which Dpp signaling controls tissue growth. Identification of Brinker binding sites in Wing imaginal discs cells

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Project description:RNA-seq was performed to confirm the effect of dMyc overexpression or RNAi repression on conserved Myc targets in the Drosophila wing disc.

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