Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic analysis of tudor[1] mutant Drosophila brain and ovaries

ABSTRACT: Transcriptomic analysis of tudor[1] mutant Drosophila brain and ovaries

PROVIDER: PRJNA629835 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11680853.fastq.gz	Fastqsanger.gz
	SRR11680854.fastq.gz	Fastqsanger.gz
	SRR11680855.fastq.gz	Fastqsanger.gz
	SRR11680856.fastq.gz	Fastqsanger.gz
	SRR11680857.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Transcriptomic analysis of tudor[1] mutant Drosophila brain and ovaries

Project description:In Drosophila, Tudor protein and its germline partners, Piwi proteins, are expressed in the brain. However, the potential significance of Tudor in neurobiology has not been explored. Here, we test a hypothesis that Tudor is an essential regulator of post-transcriptional gene expression in the brain where it controls levels of certain RNAs required for brain functions. Specifically, transcriptome of tudor mutant brains is compared with that of wild-type brains using next-generation sequencing (RNA-Seq). The hypothesis that Tudor regulates the same genes in both brain and germline is tested by comparing the transcriptomes from tudor mutant brains and ovaries. This research aims at providing innovative outcomes which may reveal exciting commonalities between the germline and brain and may contribute to our understanding of neurodegenerative disorders and the mechanisms of learning and memory.

2020-11-27 | GSE149745 | GEO

piRNA analysis of tudor[1] mutant Drosophila brain and ovaries

Project description:piRNA analysis of tudor[1] mutant Drosophila brain and ovaries

| PRJNA629836 | ENA

piRNA and transcriptomic analysis of tudor[1] mutant Drosophila brain and ovaries

Project description:piRNA and transcriptomic analysis of tudor[1] mutant Drosophila brain and ovaries

| PRJNA629840 | ENA

piRNA analysis of tudor[1] mutant Drosophila brain and ovaries

Project description:23-29 nt Piwi-interacting RNAs (piRNAs) are crucial components of the ribonucleoprotein complexes which silence the most abundant class of mobile genetic elements in human genome, retrotransposons, in germline (germ) cells. In these cells, antisense piRNAs serve as RNA guides for Piwi proteins, base pairing with transposon RNAs which are subsequently cleaved by Piwi proteins. Germ cells belong to special class of stem cells which ultimately give rise to eggs and sperm and therefore, to next generations. Therefore, piRNAs protect next-generation genomes from devastating mutations caused by transposon insertions. Although, role of piRNAs in germ cells has been studied, functions of piRNAs and their associated proteins in somatic cells are not well understood. Importantly, Piwi proteins are expressed in the fruit fly Drosophila brain and are required for the silencing of transposable elements there, clearly indicating that Piwi-associated piRNAs are involved in this process in the brain. Furthermore, piRNAs have been implicated in the memory formation mechanisms in Aplysia brain. In addition to Piwi proteins, their associated partner, molecular scaffold Tudor protein, participates in piRNA biogenesis in germ cells and it is absolutely required for germline development. However, although tudor gene is expressed in the fly brain, its role in the central nervous system is not understood. In this study, we look at the role of Tudor as an essential player in piRNA biogenesis in Drosophila brain.

2020-11-27 | GSE149748 | GEO

Cut&Run in Drosophila melanogaster control, mutant and knockdown ovaries

Project description:H3K27me3 profiles using Cleavage under targets and Release using nuclease (Cut&Run) in control and KD Drosophila melanogaster ovaries. We examined the impact on chromatin profiles in Drosophila melanogaster ovaries in which the lid, the Sin3a, the Snr1 or the mod(mdg4) gene have been selectively knocked down by tissue-specific shRNA expression. We additionally explored H3K27me3 and H3K9me3 in control and dhd mutant ovaries either carrying or not a transgene.

2021-12-10 | GSE174250 | GEO

Cut&Run in Drosophila melanogaster control, mutant and knockdown ovaries

Project description:Cut&Run in Drosophila melanogaster control, mutant and knockdown ovaries

| PRJNA728960 | ENA

Next-generation sequencing analysis of Piwi-interacting RNAs (piRNAs) in Drosophila wild-type and bel mutant ovaries

Project description:Belle has been known to be co-localized with piRNA-related proteins at the nuage of germline cells during Drosophila oogenesis. However, its role in piRNA biogenesis remains unclear. To reveal whether Belle is involved in regulating piRNA expression, we performed next-generation sequencing analysis of small non-coding RNAs on ovaries harvested from the wild type (W1118) and trans-heterozygous bel[74407/neo30] mutant. Small RNA-seq experiments were performed on three individual ovary samples with the same genotype. For piRNA expression analysis, we performed mapping of three sets of small RNA sequencing reads for each genotype to previously identified eight distinct piRNA clusters located in four different Drosophila chromosomes (from X to 4). Analysis of the piRNA expression profiling from these piRNA cluster loci indicates that some specific piRNA populations were either upregulated or downregulated in bel mutant ovaries compared with wild-type ovaries. Furthermore, we performed systematic analysis by mapping piRNA sequencing reads to sequences of all identified Drosophila transposable elements (TEs) to classify and measure piRNA reads based on their TE targets. Among 124 TE-classified piRNA populations, 9 and 20 of them were upregulated and downregulated (≥2 folds), respectively, in bel74407/neo30 mutant ovaries compared with those from wild-type ovaries. To examine the effect of the bel[74407/neo30] mutation on the ping-pong cycle for secondary piRNA biogenesis, analysis of the ping-pong signature of piRNAs specifically mapped to the retro-element Burdock was performed. The ping-pong signature for the generation of secondary piRNAs was not significantly altered in bel mutants compared with the wild type. These results, taken together, indicate that Bel is not required for primary and secondary piRNA biogenesis, but it is involved in regulating expression of specific subsets of piRNA populations.

2017-09-01 | GSE65565 | GEO

H3K4me3 profiling in Drosophila melanogaster ovaries

Project description:Compare the distribution of H3K4me3 between two experimental conditions (WT and Germ cell specific Lid KD) in Drosophila ovaries

2019-12-11 | GSE133202 | GEO

Sequencing of RNA and footprints of wildtype and RpS5b mutant Drosophila ovaries

Project description:Sequencing of RNA and footprints of wildtype and RpS5b mutant Drosophila ovaries

| PRJNA714404 | ENA

RNA sequencing of nos-gal4>phf7ey Drosophila ovaries

Project description:Next generation sequencing of mRNA for nos-gal4>phf7ey Drosophila ovaries

2020-08-28 | GSE150213 | GEO