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RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C. elegans in Pseudomonas aeruginosa (PA14) infection

ABSTRACT: RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C. elegans in Pseudomonas aeruginosa (PA14) infection

PROVIDER: PRJNA630289 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11687205_1.fastq.gz	Fastqsanger.gz
	SRR11687205_2.fastq.gz	Fastqsanger.gz
	SRR11687206_1.fastq.gz	Fastqsanger.gz
	SRR11687206_2.fastq.gz	Fastqsanger.gz
	SRR11687207_1.fastq.gz	Fastqsanger.gz

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RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C. elegans in Pseudomonas aeruginosa (PA14) infection

Project description:Purpose: To uncover immune mediated genes and pathways by Pseudomonas aeruginosa infection that are modulated by the homeodomain PITX1/UNC-30, which plays a vital role in the GABAergic signaling in C. elegans Methods: RNA was extracted from synchronized Pseudomonas aeruginosa infected L4 stage unc-30(ok613) and WT using Qiagen extraction kits and following standard methods. The animals were grown on OP50 at 20 C and infected at 25 C. Results: RNA seq analyses shows enriched and signficant Pseudomonas aeruginosa mediated upregulated immune, neuropeptide and metabolism genes and pathways that are dependent of GABAergic signaling Conclusions: Our study uncovered GABAgergic signaling to be modulator of the innate immunity in C elegans during Pseudomonas aeruginosa infection

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Project description:Purpose: To uncover immune and longevity genes and pathways that are modulated by the homeodomain PITX1/UNC-30, which plays a vital role in the GABAergic signaling in C elegans Methods: RNA was extracted from synchronized L4 stage unc-30(ok613) and WT animals grown at 20 C using Qiagen extraction kits and following standard methods Results: RNA seq analyses shows enriched and signficant upregulated immune, neuropeptide, ageing and metabolism genes and pathways that are dependent of GABAergic signaling Conclusions: Our study uncovered GABAgergic signaling to be modulator of the innate immunity in C elegans

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