Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic analysis of neonatal supporting cells after histone modification perturbations

ABSTRACT: Transcriptomic analysis of neonatal supporting cells after histone modification perturbations

PROVIDER: PRJNA630759 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11714376.fastq.gz	Fastqsanger.gz
	SRR11714377.fastq.gz	Fastqsanger.gz
	SRR11714378_1.fastq.gz	Fastqsanger.gz
	SRR11714378_2.fastq.gz	Fastqsanger.gz
	SRR11714379_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Transcriptomic analysis of neonatal supporting cells after histone modification perturbations

Project description:At neonatal stage, sensory hair cell gene promoters and enhancers are primed by H3K4me3/me1 in neighboring supporting cells, but silenced by deacetylation and H3K27 tri-methylation. To test whether this primed-but-silenced epigenetic status leads to the repressed expression of hair cell genes in supporting cells, we FACS-purified Lfng-GFP+ supporting cells from cultured postnatal day 1 (P1) ochlear organs 48 hours after TSA (HDAC inhibitor) or GSK-343 (EZH2 inhibitor) for transcritomic analysis by RNAseq. We found that hair cell genes could be induced by either blocking of histone deacetylation or inhibition of H3K27 trimethylation, suggesting histone deacetylation and H3K27 trimethylation are required to repress hair cell gene expression in supporting cells at neonatal stage when a latent transdifferentiation potential still exists in supporting cells.

2021-07-09 | GSE150001 | GEO

RNA-seq analysis of neonatal mouse cochlear supporting cells [NonTreated_JM]

Project description:This study examined transcripts that are enriched in neonatal mouse cochlear supporting cells at postnatal day 1 and postnatal day 6. Supporting cells were purified by FACS sorting for GFP fluorescence from the cochleas of transgenic mice in which a BAC including the LFng locus drives the expression of GFP. Two replicates of GFP+ supporting cells were compared with all other cochlear cell types that were GFP-. We performed this experiment at two different ages, postnatal day 1 and postnatal day 6. mRNA profiles of supporting cells (GFP+) and all other cochlear cell types (GFP-), two replicates each, at P1 and P6 mice were generated by deep sequencing using Illumna TruSeq.

2016-07-01 | E-GEOD-83356 | biostudies-arrayexpress

Mechanistic basis of neonatal heart regeneration revealed by transcriptome and histone modification profiling

Project description:Mechanistic basis of neonatal heart regeneration revealed by transcriptome and histone modification profiling

| PRJNA510088 | ENA

RNA-seq analysis of neonatal mouse cochlear supporting cells

Project description:RNA-seq analysis of neonatal mouse cochlear supporting cells

| PRJNA325664 | ENA

RNA-seq analysis of neonatal mouse cochlear supporting cells [NonTreated_JM]

2016-07-01 | GSE83356 | GEO

RNA-seq analysis of neonatal mouse cochlear supporting cells [Treated AK]

Project description:This study examined transcripts that are enriched in neonatal mouse cochlear supporting cells at postnatal day 1 and postnatal day 6 after inhibition of the Notch signaling pathway. Cochleas from postnatal day 0 and postnatal day 5 were cultured for 24 hours in the gamma secretase inhibitor DAPT or DMSO as a vehicle control. Supporting cells were purified by FACS sorting for GFP fluorescence from the cochleas of transgenic mice in which a BAC including the LFng locus drives the expression of GFP. Two replicates of GFP+ supporting cells were compared with all other cochlear cell types that were GFP-. We performed this experiment at two different ages, postnatal day 0+24 hours culture and postnatal day 5 + 24 hours culture. (corresponding to P1 and P6). mRNA profiles of P0 and P5 supporting cells (GFP+) and all other cochlear cell types (GFP-) treated with DAPT or DMSO, two replicates each, were generated by deep sequencing using Illumna TruSeq.

2016-07-01 | E-GEOD-83340 | biostudies-arrayexpress

Transcriptomic and epigenetic analysis of neonatal supporting cells during Notch inhibition-induced transdifferentiation

Project description:Latent regeneration potential has been found in neonatal supporting cells in the organ of Corti in mouse. Upon Notch inhibition by DAPT, postnatal day 1 (P1) supporting cells transdifferentiate into hair cells. To profile the transcriptomic and epigenetic changes in supporting cells during transdifferentiation, we FACS purified supporting cells from DAPT treated cochleae for RNAseq, ATACseq and H3K27ac CUT&RUN. We found that hair cell genes were up-regulated in supporting cells after DAPT treatment and that hair cell gene induction was accompanied by epigentic activation of hair cell gene cis-regulatory elements through chromatin accessibility increase and H3K27ac accumulation, suggesting the regulatory roles of epigenetic activation of hair cell gene elements for hair cell gene expression induction.

2021-07-09 | GSE150002 | GEO

Deduction of proportional contributions of histone-modification-related DNA from various tissues in plasma

Project description:Deduction of proportional contributions of histone-modification-related DNA from various tissues in plasma

| EGAS00001006503 | EGA

RNA-seq analysis of neonatal mouse cochlear supporting cells [Treated AK]

2016-07-01 | GSE83340 | GEO

Histone Modification in Cerebral Arteriovenous Malformation

Project description:Histone Modification in Cerebral Arteriovenous Malformation

| PRJNA1027297 | ENA