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Saccharomyces cerevisiae BY4741

ABSTRACT: Rtt109 effect on replication speed via histone acetylation

PROVIDER: PRJNA631463 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11848000.fastq.gz	Fastqsanger.gz
	SRR11848100.fastq.gz	Fastqsanger.gz
	SRR11848200.fastq.gz	Fastqsanger.gz
	SRR11848001.fastq.gz	Fastqsanger.gz
	SRR11848101.fastq.gz	Fastqsanger.gz

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A role for the Saccharomyces cerevisiae Rtt109 histone acetyltransferase in R-loop homeostasis and associated genome instability

Project description:The stability of the genome is occasionally challenged by the formation of DNA-RNA hybrids and R-loops, which can be influenced by the chromatin context. This is mainly due to the fact that DNA-RNA hybrids hamper the progression of replication forks, leading to fork stalling and, ultimately, DNA breaks. Through a specific screening of chromatin modifiers performed in the yeast Saccharomyces cerevisiae, we have found that the Rtt109 histone acetyltransferase is involved in several steps of R-loop-metabolism and their associated genetic instability. On one hand, Rtt109 prevents DNA-RNA hybridization by the acetylation of histone H3 lysines 14 and 23, and on the other hand, it is involved in the repair of replication-born DNA breaks, such as those that can be caused by R-loops, by acetylating lysines 14 and 56. In addition, Rtt109 loss renders cells highly sensitive to replication stress in combination with R-loop-accumulating THO-complex mutants. Our data evidence that the chromatin context simultaneously influences the occurrence of DNA-RNA hybrid-associated DNA damage and its repair, adding complexity to the source of R-loop-associated genetic instability.

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