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Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites

ABSTRACT: Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites

PROVIDER: PRJNA632768 | ENA |

REPOSITORIES: ENA

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Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites [ChIP-chip]

Project description:Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites [ChIP-chip]

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Project description:ABSCISIC ACID INSENSITIVE3 (ABI3) is a transcription factor that is a member of the B3 domain family and is involved in seed development. To understand how it controls development, we mapped genome-wide binding sites by ChIP-chip and combined this data wi Ran Tian, Fangfang Wang, Qiaolin Zheng, Venus Niza, A. Bruce Downie and Sharyn E. Perry (2020)

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Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:S. ratti Isofemale line ED321 Heterogonic predominantly undergoes indirect development; isofemale line ED5 Homogonic predominantly undergoes direct development. Therefore, L2 stages of ED321 Heterogonic and of ED5 Homogonic are destined for indirect (i.e. L2 indirect) and direct (i.e. L2 direct) development, respectively; these sources of material were used in this comparison. To do this, for both isofemale lines, rats were infected with ED321 Heterogonic or ED5 Homogonic and faeces collected on days 5, 6, 7 and 8 p.i. and cultured for 24 h at 19oC, after which larvae were prepared with a Baermann funnel held for 6 h at 19oC, larvae were concentrated and cleaned by sucrose flotation, as above. In excess of 75,000 worms were routinely isolated from three infected hosts. The experimental design used, was to have at least three biological replicates for each sample (i.e. three independent preparations of the relevant worm samples and their RNA) and to have at least three technical replicates (i.e. independent, separate cDNA synthesis, amplification and hybridization etc.) for each biological replicate. For each hybridisation a dye-swap was used i.e. each sample to be used in a hybridisation was labelled, separately, with each of the two dyes.

2022-11-22 | E-MEXP-709 | biostudies-arrayexpress

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