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MiRNA Array of Transformed FTSECs [OCV versus CV & FAC versus UNT]

ABSTRACT: miRNA Array of Transformed FTSECs [OCV versus CV & FAC versus UNT]

PROVIDER: PRJNA632940 | ENA |

REPOSITORIES: ENA

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miRNA Array of Transformed FTSECs [OCV versus CV & FAC versus UNT]

Project description:We implementing a transcriptomic approach to identify global miRNA alterations in chronic iron exposed FTSECs. Chronic iron exposure in fallopian tube secretory epithelial cells (FTSECs) leads to alterations in oncogene expression and functional changes reminescent of early changes in ovarian cancer development (serous epithelial high grade ovarian tumors).

2021-03-24 | GSE150622 | GEO

Comparative transcription profiling upon FBLN5 overexpression in transformed COS-7 and normal CV-1 cells

Project description:A novel class of genes has recently been identified as anticancer genes, which upon ectopic overexpression selectively destroy the transformed tumour cells, while leaving the untransformed normal cells unharmed. Examples include TRAIL, PAR4 and Orctl3. We have isolated 16 novel anticancer genes of human origin through a gain of function, forward genetic screen in mammalian cells. Among these, FBLN5 was chosen for further investigation. FBLN5 is a secreted ECM glycoprotein the physiological functions of which remain largely elusive, however it is downregulated in numerous malignancies and Fibln5-/- mice exhibited elastic fibre abnormalities including cutis laxa. When transfected in the normal CV-1 cells and their SV-40 transformed counterpart COS-7 cells, FBLN5 caused extensive cell death in latter and not in the former. In order to investigate such differential effects of FBLN5, we performed transcriptional profiling of COS-7 and CV-1 cells upon FBLN5 transfection where wild type COS-7 and CV-1 cells were used as controls. We conducted exon-level expression profiles of > 540,000 transcripts including coding mRNA, long non-coding RNA, microRNA, novel transcripts and also the alternative splice variants. Briefly, FBLN5 caused downregulation of MYC regulated genes in COS-7 cells while opposite was observed in CV-1 cells. Also, FBLN5 caused upregulation of cell death related genes in COS-7 cells but not in CV-1 cells.

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Project description:Microarray experiments were performed using FAC-sorted young photoreceptors to analyze their transcriptome in comparison to remaining retinal cells at same developmental stage and retinal progenitors. For each replicate, retinae of 6 to 8 postnatal day 0 pNestin-GFP or postnatal day 4 rhoEGFP mice were dissected and FAC-sorted based on GFP expression. RNA of fractions was isolated and subsequently analyzed with microarray experiment.

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Francisella tularensis strain:FAC

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Expression profile of FAC-sorted murine retinal cells

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Expression analysis of CaMsrB2-transformed rice (Oryza sativa L. cv. ILMI) without/with Bar selection marker under drought stress conditions

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