Project description:To further identified miRNAs of BALB/c mouse lungs infected with A/Anhui/1/2013 (H7N9) (hereafter referred to as H7N9/AH1-PB2-627K(WT)) and mutant variants with PB2 amino acid substitutions (avian-like H7N9/AH1-PB2-627E and mammalian-adapted H7N9/AH1-PB2-627E/701N) , miRNA microarray was carried out.
Project description:MiRNAs are important posttranscriptional regulators in various physiological processes and their dysregulations have been found in diseases such as infection, imflammation and cancer. Here we compared the miRNA profilings between HBV-producing HepG2.2.15 and its non-HBV maternal HepG2 cell to find potential miRNAs involving in HBV expression. The result indicated that some of the deregulated miRNAs in HepG2.2.15 have been implicated in viral infection and the processes of the immune regulation. HepG2.2.15 vs. HepG2
Project description:MiRNAs are important posttranscriptional regulators in various physiological processes and their dysregulations have been found in diseases such as infection, imflammation and cancer. Here we compared the miRNA profilings between HBV-producing HepG2.2.15 and its non-HBV maternal HepG2 cell to find potential miRNAs involving in HBV expression. The result indicated that some of the deregulated miRNAs in HepG2.2.15 have been implicated in viral infection and the processes of the immune regulation.
Project description:miRNAs are small regulatory non-coding RNA molecules that influence a wide range of biological and pathological processes. Piling evidence has shown that miRNAs regulate the expression of various genes pivotal for tumor onset, progression and metastasis. Here we compared the miRNA profiling between T29(human ovarian epithelial cell immortalized with SV40T/t antigens and the human telomerase catalytic subunit) and T29H (T29 transformed with the oncogenic H-RASv12) cell lines.
Project description:The goal of this experiment was to determine gene expression changes during influenza A virus infection as the result of expression influenza virus inducible miRNAs in A549 cells.
Project description:As accumulating evidence suggests cancer cells exhibit stem-like properties for advanced diseases and cancer stem cells (CSCs) have been identified in various tumor types, revisiting present knowledge toward CSCs is essential. The identification of exosome-loaded miRNAs responsible for stemness and chemoradioresistance of CSCs should eventually leadd to development of novel therapeutic approaches.
Project description:The goal of this experiment was to determine gene expression changes during influenza A virus infection as the result of expression influenza virus inducible miRNAs in A549 cells. The gene expression profiling experiment was performed with 4 groups (mock infected, influenza A virus infected, influenza A virus infected in the presence of exogenous miR-141, miR-374b, miR-449b, miR-518b, and miR-1263, and influenza A virus infected in the presence of exogenous miR-147b, miR-190b, miR-199a, miR-512-5p, and miR-874 with 3 biological replicates for each group. Total RNA was purified from A549 cells that were mock infected or infected with influenza A virus (A/WSN/33, 5pfu/cell) alone or in the presence of miRNA mimics 10 hours after treatment.
Project description:Colorectal cancer (CRC) is one of the most malignant cancers with high morbidity and mortality. MicroRNAs (miRNAs) are small noncoding RNAs that affect biological processes by binding to mRNAs and regulating their expression, and epigenetic alterations including miRNA dysregulation are significantly involved in CRC development. Here we report significantly up-regulated as well as down-regulated miRNAs in CRC tissues compared with their matched non-tumoral tissues.