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Scar-free healing and axon regeneration after spinal cord injury in neonatal mice is orchestrated by microglia

ABSTRACT: Scar-free healing and axon regeneration after spinal cord injury in neonatal mice is orchestrated by microglia

PROVIDER: PRJNA633895 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11813300.fastq.gz	Fastqsanger.gz
	SRR11813301.fastq.gz	Fastqsanger.gz
	SRR11813302.fastq.gz	Fastqsanger.gz
	SRR11813303.fastq.gz	Fastqsanger.gz
	SRR11813304.fastq.gz	Fastqsanger.gz

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Scar-free healing and axon regeneration after spinal cord injury in neonatal mice is orchestrated by microglia

Project description:By utilizing single-cell analysis at different times after P2 injury(0, 3, and 5 dpi), we discovered that neonatal microglia undergo a transient activation and play at least two critical roles in scar-free healing. First, they transiently secrete fibronectin and its binding proteins, to form extracellular matrix bridges that ligate the severed ends. Second, neonatal, but not adult, microglia express a number of extracellular and intracellular peptidase inhibitors, along with other molecules involved in inflammatory resolution.

2020-10-07 | GSE150871 | GEO

Post-injury born oligodendrocytes incorporate into the glial scar and contribute to the inhibition of axon regeneration

Project description:Pathologies of the central nervous system (CNS) white matter often result in permanent functional deficits because mature mammalian projection neurons fail to regenerate long-distance axons after injury. A major barrier to axonal regenerative research is that the CNS axons that regenerate in response to experimental treatments stall growth before reaching their post-synaptic targets. Here, we test the hypothesis that interaction of regenerating axons with live oligodendrocytes, which were absent during developmental axon growth, stalls axonal growth. To test this hypothesis, first, we used single cell RNA-seq (scRNA-seq) and immunohistological analysis to investigate whether post-injury born oligodendrocytes incorporate into the glial scar after optic nerve injury. Then, we administered demyelination-inducing cuprizone (used in studies of multiple sclerosis), concurrently with the stimulation of axon regeneration by Pten knockdown (KD) in projection neurons after optic nerve injury. We found that post-injury born oligodendrocyte lineage cells incorporate into the glial scar, where they are susceptible to the demyelination diet treatment, which reduced their presence in the glial scar. We further found that the demyelination diet enhanced Pten KD-stimulated axon regeneration, and localized injection of cuprizone promoted axon regeneration. We also present a website for comparing the gene expression of scRNA-seq-profiled optic nerve oligodendrocyte lineage cells under physiological and pathophysiological conditions. Annotation of oligodendrocyte lineage cell subtypes, and normal or injured condition, are indicated in the last two rows of the cell matrix CSV file.

2023-03-27 | GSE225410 | GEO

Deregulated immune signature orchestrated by FOXM1 impairs human diabetic wound healing

Project description:Deregulated immune signature orchestrated by FOXM1 impairs human diabetic wound healing

| PRJNA555081 | ENA

A novel role for SALL4 during scar-free wound healing in axolotl

Project description:Salamanders, such as the Mexican axolotl, are some of the few vertebrates fortunate in their ability to regenerate diverse structures after injury. Unlike mammals they are able to regenerate a fully functional spinal cord after injury. Throughout human life, many cells and certain tissues, such as hair follicle stem cells and liver, can be continuously replaced to maintain functional integrity in response to normal daily wear and tear. However, the human response to more serious tissue damage is limited to relatively primitive wound healing, whereby collagenous scar tissue fills the injury site, assuring the tissue’s structural integrity but often resulting in a debilitating loss of functionality. In contrast many vertebrates, including axolotls, have remarkable regenerative capacity including the functional regeneration of full thickness wounds. Here, we have identified a novel role for SALL4 in regulating collagen transcriptional after injury that is essential to ensure perfect skin regeneration in axolotl.

2017-03-07 | GSE79299 | GEO

Metabolic Reprogramming and Reliance in Human Skin Wound Healing

Project description:Impaired skin wound healing is a significant global health issue, especially among the elderly. Wound healing is a well-orchestrated process involving the sequential phases of inflammation, proliferation, and tissue remodeling. Although wound healing is a highly dynamic and energy-requiring process, the role of metabolism remains largely unexplored. By combining transcriptomics and metabolomics of human skin biopsy samples, we mapped the core bioenergetic and metabolic changes in normal acute as well as chronic wounds in elderly subjects. We found upregulation of glycolysis, the tricarboxylic acid cycle, glutaminolysis, and β-oxidation in the later stages of acute wound healing and in chronic wounds. To ascertain the role of these metabolic pathways on wound healing, we targeted each pathway in a wound healing assay as well as in a human skin explant model using metabolic inhibitors and stimulants. Enhancement or inhibition of glycolysis and, to a lesser extent, glutaminolysis had a far greater impact on wound healing than similar manipulations of oxidative phosphorylation and fatty acid β-oxidation. These findings increase the understanding of wound metabolism and identify glycolysis and glutaminolysis as potential targets for therapeutic intervention.

2024-07-02 | MTBLS3315 | MetaboLights

The effects of scar resection in chronic complete spinal cord injury(SCI) and the effects of scar resection combined with decellularized hydrogel (scaffold)

Project description:This study examines the molecular effects of surgical scar resection in　complete chronic spinal cord injury (SCI) through RNA sequencing. Results show that scar resection alters gene expression, with downregulation of axonal growth inhibitors and upregulation of neuroprotective genes. Additionally, the application of a decellularized extracellular matrix (dECM) hydrogel as a scaffold promoted angiogenesis. Gene Ontology analysis indicated enrichment of immune-related processes, suggesting that scar resection improves the spinal cord microenvironment for neural repair. These findings indicate the potential of combining scar resection with scaffolding to support recovery after chronic complete SCI.

2024-10-08 | GSE278491 | GEO

Microarray profiling analysis of microRNAs expression in Hypertrophic scar

Project description:Excessive repair after burn or trauma will lead to the formation of pathological scar. TGF-β1 is a powerful growth factor after wound healing. It is considered to be a key regulator of HS and various fibrotic diseases. MicroRNAs (miRNAs) can widely participate in the pathophysiological processes of various diseases by participating in post transcriptional gene regulation. At present, there is no research report on miR-361 and hypertrophic scar. This study found that miR-361 in HS is down-regulated. MiR-361 can inhibit the proliferation of HS fibroblasts and promote their apoptosis by inhibiting TGF-β1. Moreover, miR-361 can inhibit the formation of rabbit ear scar by inhibiting the expression of TGF-β1.

2021-11-22 | GSE189134 | GEO

Transcriptional profiling of a wound healing process in skin and oral mucosa

Project description:When compared to skin, oral mucosal wounds heal rapidly and with reduced scar formation. This study used an Affymetrix microarray platform to compare the transcriptomes of oral mucosa and skin wounds in order to identify critical differences in the healing response at these two sites. Using microarrays, we explored the differences in gene expression in skin and oral mucosal wound healing in a murine model of paired equivalent-sized wounds. Samples were examined from day 0 to day 10 and spanned all stages of the wound healing process. Unwounded matched tissue was used as a control. Tissue samples collected at each post-wounding time point, as well as control samples, were represented by 3 biological replicates.

2010-07-20 | E-GEOD-23006 | biostudies-arrayexpress

Hypoxia and Foxn1 alter dermal fibroblasts proteomics signature to redirect scar-less to scar-forming skin wound healing in Foxn1-/- mice

Project description:Hypoxia is one of the factors that govern reparative vs regenerative skin wound healing. Data-independent acquisition (DIA) experiment was carried out to study the effect of hypoxia and Foxn1 on mice dermal fibroblast proteomics signature and skin wound healing of Foxn1-deficient (Foxn1-/-) mice.

2024-06-20 | PXD041939 | Pride

Expression data from mouse skin scar after wounding

Project description:Using a TCF reporter mouse which contain a LacZ gene downstream of a c-fos minimal promoter and three consensus TCF-binding motifs, we wounded skin and harvested the scar tissue one week post wounding. Healing tissue sorted to group of cells : β-Gal+ cells (R) expression data with β-Gal- cells (L) , an analysis of gene expression was performed using Partek Genotyping Suite and Ingenuity Systems Software.

2011-07-26 | GSE30913 | GEO

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