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ECLIP of mouse Asterix/Gtsf1 transfected in mouse P19 embryonal teratoma cells

ABSTRACT: eCLIP of mouse Asterix/Gtsf1 transfected in mouse P19 embryonal teratoma cells

PROVIDER: PRJNA634751 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11837400_1.fastq.gz	Fastqsanger.gz
	SRR11837400_2.fastq.gz	Fastqsanger.gz
	SRR11837401_1.fastq.gz	Fastqsanger.gz
	SRR11837401_2.fastq.gz	Fastqsanger.gz
	SRR11837402_1.fastq.gz	Fastqsanger.gz

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eCLIP of mouse Asterix/Gtsf1 transfected in mouse P19 embryonal teratoma cells

Project description:Asterix/Gtsf1 has been previously been shown to be involved in the silencing of retrotransposons in animal germ cells through the piRNA pathway. Using eCLIP and a custom informatic pipeline, we found that Asterix/Gtsf1 binds directly to RNAs and preferentially interacts with tRNAs as a class. Given the role of tRNAs as primers for Long Terminal Repeat (LTR) transposons, this work implicates Asterix/Gtsf1 as exploiting tRNA dependence to identify transposon transcripts and promote piRNA silencing.

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RIP-seq of mouse Asterix/Gtsf1 expressed in Sf9 cells

Project description:Asterix/Gtsf1 has been previously been shown to be involved in the silencing of retrotransposons in animal germ cells through the piRNA pathway. During early stages of purification of mouse Gtsf1 protein in Sf9 cells for structural studies, we found that the protein was co-purifying with endogenous nucleic acids. We first characterized those RNAs by next-generation sequencing (this dataset) and then later investigated its RNA-binding in more relevant cell culture systems. In each case, we found Asterix/Gtsf1 was binding tRNAs. Given the role of tRNAs as primers for Long Terminal Repeat (LTR) transposons, this work implicates Asterix/Gtsf1 as exploiting tRNA dependence to identify transposon transcripts and promote piRNA silencing.

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Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons

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Profiling of H3K9cr and H3K9ac in the P19 embryonal carcinoma cells

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