Project description:Rbfox2 is required for maintaining hepatic metabolic homeostasis, here we carry out RNAseq to of WT and aged matched littermate Rbfox2 knockout mice under high fructose in order to determine changes in alternative splicing and mRNA expression caused by knockout of this splicing factor in this metabolic environment.
Project description:Rbfox2 is required for maintaining hepatic metabolic homeostasis. Here we carry out RNAseq to of WT and aged matched litter mate Rbfox2 knockout mice under CD or HFD in order to determine changes in alternative splicing and mRNA expression caused by knockout of this splicing factor in the context of metabolic health and disease.
Project description:We perform scRNA-seq in the livers of C57BL/6 mice fed a CD-HFD or control diet for 3 months (NAFLD), 6 months (NASH) or 15 months (HCC)
Project description:We perform scRNA-seq in the livers of C57BL/6 mice fed a CD-HFD for 15 months. Starting from the 13M, mice were treated with FABP5 inhibitor to ameliorate HCC.
Project description:We used RNA-seq to measure mRNA levels of livers and liver tumors from mice treated with or without the chemical carcinogen diethylnitrosamine (DEN) and fed CD or obesogenic WD, with the aim to explore how metabolism in hepatic tissues and tumors (where present) is affected by diet, carcinogen treatment and tumor development. We used the RNA-seq data for differential gene expression analysis, and for the reconstruction and constraining of genome-scale metabolic models in order to estimate metabolic changes across tissues under the various treatment conditions.
Project description:We generated a global analysis of Rbfox2 splicing regulation combined with a highly specific, single nucleotide-resolution Rbfox2 RNA binding map. We found that Rbfox2 regulates the splicing and expression of many previously unknown targets, and particularly a number of RNA binding proteins (RBPs), by modulating alternative splicing coupled-NMD. Based on our observations of RBP-Rbfox2 co-regulation with a polarity predicted by Rbfox2 binding, we propose a model whereby Rbfox2 tunes autoregulatory splicing events to control RBP expression levels and in turn alter their respective splicing networks. iCLIP for epitope-tagged Rbfox2 and control untagged Rbfox2; RNAseq of control and Rbfox2 knockdown in mouse embryonic stem cells
Project description:Gene expression in livers of male wild-type (WT) and OGG1-deficient (Ogg1-/-) mice fed either a chow diet or a high-fat diet (HFD) were examined. Mice were fed the diet for 10 weeks prior to tissue collection and were 22 weeks of age at the time of tissue collection.