Variation in gene expression between two Sorghum bicolor lines differing in innate immunity response
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA637509 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR11932513.fastq.gz | Fastqsanger.gz | |||
| SRR11932514.fastq.gz | Fastqsanger.gz | |||
| SRR11932515.fastq.gz | Fastqsanger.gz | |||
| SRR11932516.fastq.gz | Fastqsanger.gz | |||
| SRR11932517.fastq.gz | Fastqsanger.gz |
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Project description:Comparative RNA-seq analysis of MAMP triggered gene expression in two sorghum bicolor lines, BTx623 and SC155-14E, revealed a clear transcriptional response to elicitation with the microbe associated molecular pattern (MAMPs) flagellin (flg22) or chitin elicitation.
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Project description:Intercellular and systemic signalling is an essential hallmark of multicellular life. Encounter of pathogens occurs locally but triggers organ scale and organismic immune responses. In plants, elicitor perception provokes rapid, systemically expanding Ca2+ and H2O2 second messenger signals conferring innate immunity. However, the underlying components and mechanisms for enabling and sustaining signal propagation have remained elusive. Here, we report how plants achieve perpetuated Ca2+/H2O2 signal proliferation and simultaneously avoid disturbance of ongoing cellular signalling along the path of response dissemination. We identify a CBL1/CIPK26/CPK5 Ca2+ sensing/phosphorylation module from Arabidopsis thaliana as crucial for systemic signal propagation. This bi-kinase signalling module becomes Ca2+-sensitized and super-activated through mutual trans-phosphorylation and in consequence imposes a cooperative and enhanced phosphorylation pattern on the RBOHD NADPH oxidase causing maximized H2O2 generation. Biochemical, genetic and Ca2+-biosensorics analyses reveal that RBOHD phosphorylation by both kinases is essential for elicitor triggered systemic Ca2+ signalling and innate immunity manifestation. Notably, combined CIPK26/CPK5 action dramatically lowers the Ca2+ concentration threshold of RBOHD activation, and phosphorylation of RBOHD Ser343/347 provides a complementary cis-acting substrate-sensitisation/activation mechanism through determining its Ca2+ binding efficiency. These dual signalling sensitisation and actuation mechanisms maximize signal proliferation at minimized Ca2+ signal intensity to obviate interference of long distance and cellular Ca2+ signalling.
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Project description:The outcome of viral infection is extremely heterogeneous at the cellular level, and infected cells only sometimes activate innate immunity. Here we assess how the genetic variation inherent in viral populations contributes to this heterogeneity. We do this by developing a new approach to determine both the cellular transcriptome and full-length sequences of all viral genes in single influenza-infected cells. Infections that activate an innate-immune response in single cells are associated with viral defects that include amino-acid mutations, internal deletions, and failure to express key genes. However, immune activation remains stochastic in cells infected by virions with these defects, and sometimes occurs even in cells infected by virions that express unmutated copies of all genes. Our work shows that the genetic variation present in influenza virus populations substantially contributes to but does not fully explain the heterogeneity in infection outcome and immune activation in single infected cells.
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