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Single-cell transcriptome analysis of endothelial cell heterogeneity under laminar shear stress

ABSTRACT: Single-cell transcriptome analysis of endothelial cell heterogeneity under laminar shear stress

PROVIDER: PRJNA637536 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11932654.fastq.gz	Fastqsanger.gz
	SRR11932655.fastq.gz	Fastqsanger.gz
	SRR11932656.fastq.gz	Fastqsanger.gz
	SRR11932657.fastq.gz	Fastqsanger.gz
	SRR11932658.fastq.gz	Fastqsanger.gz

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Single-cell transcriptome analysis of endothelial cell heterogeneity under laminar shear stress

Project description:Heterogeneity of endothelial cells that form the innermost layer of all vessels is critical for vascular sprouting and angiogenesis. After new vessels form, endothelial cell heterogeneity is believed to be gradually lost as vessels respond to flow-mediated signals, mature, remodel and become homeostatic. However, whether and at what level endothelial cell lost heterogeneity is poorly understood. Here we investigated heterogeneity change of endothelial cells under homeostatic laminar shear tress in comparison to static cultures.

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HUVECs cultured under normal or high laminar shear stress

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Project description:Laminar shear stress due to constant blood flow is known to play a critical role in maintaining vascular health. In contrast, endothelial cell senescence appears to be closely associated with the incidence of vascular disorder. In an attempt to identify functional biomarkers for age-related vascular health/disease, the present study investigated differential gene expression of young and senescent human umbilical vein endothelial cells (HUVECs) under static and laminar shear stress. We used a cDNA microarray method to compare gene expression profiles of young and senescent HUVECs under static and laminar shear stress conditions. Experiment Overall Design: Senescent cells were prepared by continuous subculture in vitro, and a cone-and-plate device was used to impose laminar shear stress onto cells. Young and senescent cells were exposed to laminar shear stress or maintained under static conditions. Total mRNA was extracted and gene expression profiles were analyzed by cDNA microarray.

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