Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNA-Seq analysis of the human embryo dorsal aorta

ABSTRACT: Single cell RNA-Seq analysis of the human embryo dorsal aorta

PROVIDER: PRJNA637576 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11934366_1.fastq.gz	Fastqsanger.gz
	SRR11934366_2.fastq.gz	Fastqsanger.gz
	SRR11934367_1.fastq.gz	Fastqsanger.gz
	SRR11934367_2.fastq.gz	Fastqsanger.gz
	SRR11934368_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Single cell RNA-Seq analysis of the human embryo dorsal aorta

Project description:Single cell RNA-Seq exploration of the human dorsal aorta, the hematopoietic stem cell (HSC) developmental niche, to explore heterogeneity of the HSC microenvironment and elucidate lineage trajectories of nascent hematopoietic stem/progenitor cells (HSPCs) from endothelium.

2020-09-17 | GSE151876 | GEO

RNA-Seq analysis of sorted hematoendothelial populations from the human embryo dorsal aorta

Project description:This dataset contains RNA-Seq data for sorted hematoendothelial subpopulations from the ventral (AoV) and dorsal (AoV) portions of the CS15-16 human embryo dorsal aorta. This includes endothelial (VC+CD45-), Hematopoietic Stem/Progenitor Cells (VC+CD45+) and more mature hematopoietic cells (VC-CD45+) from both the AoV and AoD.

2020-09-17 | GSE151874 | GEO

RNA-Seq analysis of sorted hematoendothelial populations from the human embryo dorsal aorta

Project description:RNA-Seq analysis of sorted hematoendothelial populations from the human embryo dorsal aorta

| PRJNA637575 | ENA

LCM-Seq analysis of microscopically defined human embryo HSC microenvironment

Project description:This dataset contains RNA-Seq data for cells within spatially defined subdomains of the human embryo dorsal aorta, subdissected from tissue sections using laser capture microdissection. This dataset explores dorsal-ventral polarisation across the dorsal aorta, the site of hematopoietic stem cell emergence.

2020-09-17 | GSE151875 | GEO

Single cell profiling of hemogenic endothelium and its surrounding niche in the mouse dorsal aorta

Project description:The first hematopoietic stem cells originate from hemogenic endothelium (HE), that trans-differentiate into the lumen to form hematopoietic clusters. The molecular mechanisms driving this transition are only poorly understood. Here, we performed single cell RNA-seq profiling of HE cells utilising a RUNX1 and GFI1 reporter mouse line.This allowed for a detailed characterisation of HE cells during endothelial-to-hematopoietic transition. Additionally we profiled the cells surrounding the dorsal aorta. These dataset present a comprehensive profile at single cell resolution of cells invovled in the endothelial-to-hematopoietic transition and the surrounding dorsal aorta niche.

2021-09-21 | GSE150412 | GEO

Parallel spatial transcriptomics, single-cell and bulk RNA-Seq analysis of the human embryonic HSC niche, the dorsal aorta

Project description:Haematopoietic stem cells (HSCs) first emerge in the embryonic aorta-gonad-mesonephros (AGM) region. Studies on model organisms defined intersecting signalling pathways that converge to promote HSC emergence predominantly in the ventral domain of the dorsal aorta. Much less is known about mechanisms driving HSC development in human. Here, to identify secreted signals underlying human HSC development, we combined spatial transcriptomics analysis of dorso-ventral polarised signalling in the aorta with gene expression profiling of sorted cell populations and single cells. Our analysis revealed a subset of aortic endothelial cells with downregulated arterial signature and a predicted lineage relationship with the emerging HSC/ progenitor population. Analysis of the ventrally polarised molecular landscape identified endothelin 1 as an important secreted regulator of human HSC development. The obtained gene expression datasets will inform future studies on mechanisms of HSC development in vivo and on the generation of clinically relevant HSCs in vitro.

2020-09-17 | GSE151877 | GEO

Transcription profiling of mouse dorsal aorta derived mesangioblast clone comparison

Project description:Mouse dorsal aorta from E9.5 mouse embryos (C57BL6) was grown as an explant culture as described elsewhere (De Angelis et al., 1999). After 5 days the explant was dissociated into a single cell suspension, and plated at limiting dilution on a feeder layer of Mitomycin C-treated primary embryonic mouse fibroblasts, in 96 multiwell plates in complete medium, RPMI 20% Fetal Calf Serum. After 1 week, mesangioblast clones appeared approximately 2-4% of the wells. When the clones had grown to approximately 103 cells they were passed on gelatin-coated dishes, and further expanded. Clones D16 and D351 were used for microarray analysis.

2008-06-13 | E-GEOD-552 | biostudies-arrayexpress

Transcription profiling of mouse dorsal aorta derived mesangioblast clone comparison- U74B

2008-06-13 | E-GEOD-554 | biostudies-arrayexpress

Transcription profiling of mouse dorsal aorta from E9.5 mouse embryos (C57BL6) derived mesangioblast clone comparison

2008-06-13 | E-GEOD-555 | biostudies-arrayexpress

Single cell profiling of hemogenic endothelium and its surrounding niche in the mouse dorsal aorta

Project description:Single cell profiling of hemogenic endothelium and its surrounding niche in the mouse dorsal aorta

| PRJNA632005 | ENA