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Expression profile of the 22Rv1 cells overexpressing ABHD5

ABSTRACT: Expression profile of the 22Rv1 cells overexpressing ABHD5

PROVIDER: PRJNA637888 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11943600.fastq.gz	Fastqsanger.gz
	SRR11943601.fastq.gz	Fastqsanger.gz
	SRR11943602.fastq.gz	Fastqsanger.gz
	SRR11943603.fastq.gz	Fastqsanger.gz
	SRR11943604.fastq.gz	Fastqsanger.gz

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Expression profile of the 22Rv1 cells overexpressing ABHD5

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Protein expression profile of miR-33b overexpressing ES-2 cells upon OCM treatment

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22Rv1 in vivo progression model

Project description:Purpose: Even in last stage of metastatic castration-resistant prostate cancer, androgen receptor (AR) signaling remains active.To derive high metastatic prostate cancer (PCa), we labeled AR-positive but castration-resistant 22Rv1 PCa cells with luciferase gene (22Rv1-Luc2) and these cells were orthotopically implanted in mouse prostate for spontaneous progression. Methods: 2 × 10^5 of luciferase-expressing 22Rv1 cells (22Rv1-Luc2) cells were implanted in the anterior prostate of nude mice. After 12-14 weeks, the host mice were necropsied and the metastases from lumbar lymph nodes and primary tumors were dissected under laminar flow. Tumor tissues were minced using sterile scalpels and further digested with Collagenase D for 1 h. The lymph node metastatic cancer cells, named 22Rv1-M1, were orthotopically reimplanted in nude mice. At 12 weeks, the secondary metastases were isolated in the lumbar lymph nodes and designated as 22Rv1-M2 cells. Suspension of 1 × 10^6 22Rv1-M2 cells in DPBS was injected into nude mice through the tail vein, and mice developed metastases (22Rv1-M3) after 6 week. This procedure was repeated once to attain the 22Rv1-M4. Results: 22Rv1-derived metastatic cell lines exhibit increased in vitro and in vivo invasion activity as the progression from 22Rv1 to M4. Transcriptomic analysis of genome-wide gene expression in the M4 tumors reveal the unique gene expression profile compared to 22Rv1 tumors. Conclusions: Transcriptomic data provide the gene network for decoding the mechanism of PCa metastasis.

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Protein profiles following miRNA-23c and -4328 overexpression in prostate cancer cell lines, PC-3 and 22Rv1

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Gene expression profile of podocalyxin-like protein 1(PODXL) overexpressing and GFP overexpressing control embryonic stem cells

Project description:Gene expression profile of podocalyxin-like protein 1(PODXL) overexpressing and GFP overexpressing control embryonic stem cells

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Expression profile of HER2-overexpressing CHO cells treated with trastuzumab and pertuzumab

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