Project description:Our aim was to study the immune system of a Mediterranean sea urchin Paracentrotus lividus. Using a label free quantitative mass spectrometry approach we analyzed how bacterial lipopolysaccharide (LPS) is able to modulate the coelomocytes proteome and effect its cellular pathways such as cytoskeleton reorganization, stress and energetic homeostasis.
Project description:We sampled skin and blubber from 6 fin whale (Balenoptera physalus) individuals living in the northern Mediterranean Sea. Blubber was analyzed for Organochlorines levels while genomic DNA extracted from the skin of the animals with the lowest (mean value = 19 µg/g lipid basis, l.b.) (group 1, n=3) and the highest (mean value = 53 µg/g l.b.) (group 2, n=3) levels of contaminants were used for DNAm profiling through reduced representation bisulfite sequencing (RRBS).