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Streptomyces sp.

ABSTRACT: Sequencing of phytopathogenic Streptomyces

PROVIDER: PRJNA640830 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR12066381_1.fastq.gz	Fastqsanger.gz
	SRR12066381_2.fastq.gz	Fastqsanger.gz
	SRR12066382_1.fastq.gz	Fastqsanger.gz
	SRR12066382_2.fastq.gz	Fastqsanger.gz

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Streptomyces sp. M7 LC-MS - Streptomyces sp. M7 has demonstrated ability to remove lindane from culture media and soils

Project description:Streptomyces sp. M7 has demonstrated ability to remove lindane from culture media and soils. In this study, we used MS-based label-free quantitative proteomic to understand lindane degradation and its metabolic context in Streptomyces sp. M7. We identified the proteins involved in the up-stream degradation pathway. Our results demonstrated that mineralization of lindane is feasible since proteins from an unusual down-stream degradation pathway were also identified. Degradative steps were supported by an active catabolism that supplied energy and reducing equivalents in the form of NADPH. This is the first study in which degradation steps of an organochlorine compound and metabolic context are elucidate in a biotechnological genus as Streptomyces. These results serve as basement to study other degradative actinobacteria and to improve the degradation processes of Streptomyces sp. M7.

2018-08-10 | PXD010607 | Pride

Genome-wide binding site profiling of NdgR in Streptomyces coelicolor

Project description:We identified genome-wide binding regions of NdgR in Streptomyces coelicolor using chromatin immunoprecipitation sequencing (ChIP-seq). We constructed 6×myc-tagged NdgR strain using homologous recombination with myc-tagging vector. Analysis of the sequencing data aligned to Streptomyces coelicolor genome database (NC_003888).

2014-12-29 | GSE59010 | GEO

Examination of polyester-polyurethane degradation by Streptomyces sp. PU10

Project description:This study aimed to investigate the variations in the protein composition of Streptomyces sp. PU10 when cultivated with either Impranil (polyestere-polyurethane) or glucose as the carbon source. We analyzed both the intracellular and extracellular protein fractions to gain insights into the intricate processes involving PU degradation, intermediate metabolic pathways in PU degradation, and the connection between primary and secondary metabolism within Streptomyces sp. PU10.

2024-03-29 | PXD047534 | Pride

Ribosome profiling of Streptomyces griseus NBRC13350 and Streptomyces venezuelae ATCC15439

Project description:We performed ribosome profiling which is the deep-sequencing of mRNA fragments protected by translating ribosome for two Streptomyces species through different growth phases to provide the translatome data

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The genome of Streptomyces rimosus shows a novel structure compared to other Streptomyces

Project description:This study compared the genome of Streptomyces rimosus rimosus against that of Streptomyces coelicolor. It also compared 4 strains with changes in oxytetracycline production and derived from G7, the type strain, against G7. Keywords: Comparative genomic hybridization

2008-01-11 | GSE10126 | GEO

Streptomyces sp. MB42 proteomics response to catechin, dopamine, phenylephrine

Project description:Streptomyces sp. MB42 produces antimicrobial compound under the pressence of specific compounds. This experiment is to see which gene cluster upregulated during the treatment of target compound.

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Proteomic study of Streptomyces sp. MC1 exposed to Cr(VI) and phenanthrene

Project description:The aim of this work was to understand the response of Streptomyces sp. MC1 when exposed to Cr(VI) and phenantrene.

2020-11-25 | PXD018877 | Pride

An Arabidopsis Mutant Resistant to Thaxtomin A, a Cellulose Synthesis Inhibitor from Streptomyces Sp

Project description:Thaxtomin A is a phytotoxin produced by Streptomyces scabies and other Streptomyces species, the causative agents of common scab disease in potato and other taproot crops. At nanomolar concentrations, thaxtomin causes dramatic cell swelling, reduced seedling growth, and inhibition of cellulose synthesis in Arabidopsis. We identified a mutant of Arabidopsis, designated txr1, that exhibits increased resistance to thaxtomin as a result of a decrease in the rate of toxin uptake. The TXR1 gene was identified by map-based cloning and found to encode a novel, small protein with no apparent motifs or organelle-targeting signals. The protein, which has homologs in all fully sequenced eukaryotic genomes, is expressed in all tissues and during all developmental stages analyzed. Microarray transcript profiling of some 14,300 genes revealed two stomatin-like genes that were expressed differentially in the txr1 mutant and the wild type. We propose that TXR1 is a regulator of a transport mechanism A genetic modification design type is where an organism(s) has had genetic material removed, rearranged, mutagenized or added, such as knock out. Keywords: genetic_modification_design

2005-12-20 | GSE3873 | GEO

Project description:Sequencing of phytopathogenic Streptomyces

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RNA sequencing to identify differences between two E. coli strains that respond differently to volatiles of Streptomyces sp.

Project description:The goals of this study are to compare the transcriptomic response of a E. coli strain with a mutation in the promotor region of the outer membrane porins regulator ompR-envZ that has an increased resistance to ammonia released by a Streptomyces strain.

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