Project description:Transcriptional profiling of the jejunum mucosa with 1.5 fold-change reporter genes in comparing control black-boned chickens under normal temperature (NT) conditon with heat-stress treated black-boned chickens under high temperature (HT) condition. Goal was to determine the differentially expressed genes (DEGs) in co-family black-boned chickens exposure to heat stress based on global chicken gene expression.
Project description:Transcriptional profiling of the jejunum mucosa with 1.5 fold-change reporter genes in comparing control black-boned chickens under normal temperature (NT) conditon with heat-stress treated black-boned chickens under high temperature (HT) condition. Goal was to determine the differentially expressed genes (DEGs) in co-family black-boned chickens exposure to heat stress based on global chicken gene expression. Two-condition experiment, HT vs. NT Treatment. Biological replicates: 3 control replicates, 3 heat stressed replicates.
Project description:High throughput sequencing technology was used to sequence the transcriptome of breast muscle tissue of control group and test group IV, the results of bioinformatics analysis showed that 417 mRNAs were differentially expressed between control group and test group IV (fold change ≥ 2.0, P < 0.05). Among them, 160 mRNAs presented upregulated and 257 were downregulated. The differentially expressed mRNAs were mainly involved in Melanogenesis, Calcium signaling pathway, Wnt signaling pathway, mTOR signaling pathway, and Vascular smooth muscle contraction.
Project description:This work was to study the transcriptome profiles in the skin of chickens with black versus white skin using high-throughput RNA deep-sequencing technology, to investigate the different expression profiles of the genes involved in skin pigmentation, then look for the main differences between black and white skin colors in Lueyang chickens.