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Transcriptional Sequencing of Epicardium-Derived Cells from the Mouse Embryo

ABSTRACT: Transcriptional Sequencing of Epicardium-Derived Cells from the Mouse Embryo

PROVIDER: PRJNA642156 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12097485.fastq.gz	Fastqsanger.gz
	SRR12097486.fastq.gz	Fastqsanger.gz
	SRR12097487.fastq.gz	Fastqsanger.gz
	SRR12097488.fastq.gz	Fastqsanger.gz
	SRR12097489.fastq.gz	Fastqsanger.gz

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Transcriptional Sequencing of Epicardium-Derived Cells from the Mouse Embryo

Project description:The goal of this study was to perform transcriptional profiling of epicardium-derived cells, compared to non epicardium-derived cells, obtained from the embryonic heart, to interrogate transcriptional mechanisms that control epithelial to mesenchymal transition.

2020-06-27 | GSE153367 | GEO

Single Cell Transcriptional Sequencing of Epicardium-Derived Cells and Endothelial Cells During Cardiac Development

Project description:The goal of this study was to perform transcriptional profiling of single epicardium-derived cells during early cardiac development. Epicardium enriched Wilms Tumor Gene 1 (Wt1) genetic lineage tracing mouse line (Wt1CreERT2/+; R26mTmG) was utilized to collect fluorescently labeled epicardial cells at embryonic day (E) 12.5 and following epicardial-to-mesenchymal transition at E16.5. Additionally, single cell transcriptional profiling was performed in endothelial cells that were collected from Wt1CreERT2/+ mice and Wt1CreERT2/+;MRTF-A-/-;MRTF-Bflox/flox to generate myocardin-related transcription factor A and B gene deletions in the epicardium and evaluate the impact of deleting a mechanosensitive gene program on coronary vasculature development at E14.5. This transcriptional data provides a resource to determine relative mRNA changes in epicardium-derived cells and endothelial cells during significant stages of cardiac development.

2021-05-27 | GSE154715 | GEO

scRNA-seq of E15.5 epicardium and epicardium-derived cells

Project description:scRNA-seq of E15.5 epicardium and epicardium-derived cells

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scRNA-seq of E15.5 epicardium and epicardium-derived cells

Project description:The embryonic epicardium provides a source of multipotent progenitors for cardiac lineages, including pericytes, fibroblasts and smooth muscle cells. However, it remains unclear if the epicardium comprises of heterogeneous progenitors or if epicardial cells are multipotent. Using scRNA-seq, we analysed the expression profile of epicardial and epicardial-derived cells at E15.5 and found no evidence of discrete epicardial sub-compartments. Our findings also support the notion that epicardial fate is specified after epithelial-mesenchymal transition, not pre-determined.

2020-04-30 | GSE145832 | GEO

Single Cell Transcriptional Sequencing of Epicardium-Derived Cells and Endothelial Cells During Cardiac Development

Project description:Single Cell Transcriptional Sequencing of Epicardium-Derived Cells and Endothelial Cells During Cardiac Development

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EPICARDIOID SINGLE-CELL GENOMICS UNCOVER PRINCIPLES OF HUMAN EPICARDIUM BIOLOGY IN HEART DEVELOPMENT AND DISEASE [scATAC-Seq]

Project description:Native human epicardial precursors are virtually inaccessible, as they appear in the embryo less than four weeks post-conception, at which point pregnancy may not yet be detected. Protocols have been established to generate epicardial cells and their progeny from human pluripotent stem cells in vitro. However, no current model is amenable to studying the many facets of human epicardial biology, notably epicardium formation, lineage heterogeneity, and functional crosstalk with other cardiac cell types during organ development and disease. Here, we generated human pluripotent stem cell-derived 3D heart organoids showing retinoic acid-dependent self-organization of the epicardium and myocardium, which we called epicardioids. Time course single-cell RNA sequencing revealed that epicardioids are formed through the specification of first heart field progenitors, a subset of which closely correspond to juxta-cardiac field (JCF) cells that have recently been identified in the embryonic mouse heart as novel common progenitors of myocardium and epicardium and have not yet been described and studied in humans. Analysis of chromatin accessibility by single-cell ATAC-seq additionally revealed key transcriptional programs guiding fate decisions along the epicardial lineage tree. Human epicardioids provide a unique model to gain fundamental insights into human epicardium biology and function during heart development, homeostasis, and disease.

2023-02-05 | GSE196512 | GEO

EPICARDIOID SINGLE-CELL GENOMICS UNCOVER PRINCIPLES OF HUMAN EPICARDIUM BIOLOGY IN HEART DEVELOPMENT AND DISEASE [scRNA-Seq]

2023-02-05 | GSE196513 | GEO

comparison of primary epicardium explant derived CDCs and primary Epicardial cells

Project description:The analysis showed differences of transcriptomes between primary epicardium explant derived CDCs and primary Epicardial cells directly isolated from the heart epicardium.SB-431542 was an effective TGF-b pathway inhibitor that could obviously inhibit the epicardial cells Epithelial-mesenchymal transition. SB-432542 treatment could produce some epithelial clones in the CDCs,which showed epicardiac specific expression profile, compared with the CDCs.

2022-02-18 | GSE189275 | GEO

Transcriptional profiling of embryo cryotolerance

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comparison of primary epicardium explant derived CDCs and primary Epicardial cells

Project description:comparison of primary epicardium explant derived CDCs and primary Epicardial cells

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