Project description:To comprehensively examine differences of gene expression patterns between WT and ATG5 KO HSCs/progenitors, we isolated LSK cells and conducted the microarray analysis.
Project description:VAMP7 is involved in autophagy and in exocytosis mediating neurite growth, two yet unconnected cellular pathways. Here we found that nutrient restriction and activation of autophagy stimulated axonal growth while inhibition led to multiple axons. VAMP7 KO neuronal cells showed impaired whereas ATG5 KO cells showed increased neurite growth. Secretomics identified that ER-phagy-related LC3 interacting region-containing proteins Atlastins and Reticulons were more abundant in ATG5 KO and less abundant in VAMP7 KO secretomes. The secretion of reticulon 3, LC3-II and P62 was impaired in VAMP7 KO cells. Treatment of neuronal cells with ATG5 or VAMP7 KO conditioned medium showed no effect thus the role of ATG5 and VAMP7 in neurite growth was cell autonomous. A nanobody directed against VAMP7 inhibited axonal growth induced by nutrient restriction. Furthermore, expression of the inhibitory Longin domain of VAMP7 impaired the subcellular localization of reticulon 3 in neurons. We propose that VAMP7-dependent unconventional autophagy-dependent secretion is an essential mechanism for axonal growth.
Project description:To comprehensively investigate differences of gene expression patterns between Spred1 WT and KO HSCs/progenitors, we isolated HSCs/progenitor cells and performed the microarray analysis.
Project description:Male C57BL/6 normal and ATG5-KO mouse dendritic cells exposed to interleukin-4 (IL4) and/or lipopolisaccharyde (LPS) 24 samples representing the different biological combinations between wild type and ko cells exposed to IL4 and LPS (three replicates for each)
Project description:Nfatc1 short isoform-specific KO mice are embryonic lethal. This isoform is essential for osteoclast differentiation and is responsible for the gene expression of various osteoclast markers, including the isoform itself. We used clariom s assay to explore genes showing altered expresssion during osteoclast differentiation in cultured hematopoietic progenitor cells from KO mice.
Project description:Interleukin-1β (IL-1β) drives hematopoietic stem cell (HSC) differentiation into the myeloid lineage, and enhanced IL-1β signaling plays a key role in hematological malignancies. However, little is known on the role of its endogenous regulatory cytokine, IL-1 receptor antagonist (IL-1rn), on both healthy and malignant hematopoiesis. Here, characterize transcriptomic changes at the single cell level in myeloid cells, hematopoietic stem and progenitor cells, and CD63+ mesenchymal stromal cells between C57BL/6J and IL-1rn-KO mice.