Project description:Here we describe the principles of 3D genome folding dynamics in vertebrates and show how lineage-specific patterns of genome reshuffling can result in different chromatin configurations. We (i) identified different patterns of chromosome folding across vertebrate species, (ii) reconstructed ancestral marsupial and afrotherian genomes analyzing whole-genome sequences of 10 species representative of the major therian phylogroups, (iii) detected lineage-specific chromosome rearrangements and (iv) identified the dynamics of the structural properties of genome reshuffling through therian evolution.
2022-12-08 | GSE206075 | GEO
Project description:16s rRNA sequences of different Mycoplasma species
Project description:Variations between strains have been extensively studied in human pathogens mainly because even genomically highly identical strains can cause severely different phenotypes in their hosts. Here we investigate within-species diversity in Rhabdochlamydia porcellionis a pathogen infecting terrestrial isopods and a member of the phylum Chlamydia that also includes well-known human pathogens as Chlamydia trachomatis. Using an infection assay in Sf9 insect cell cultures we could show that albeit there are only few genomic variations, the strains 15C and ZGO cause different phenotypes. To investigate potential underlying mechanisms we carried out a global gene expression analysis and could show that changes in major metabolic pathways help strain 15C to replicate more efficiently in the host cells. In addition we found that genes shown to be involved in pathogenicity and host interaction of human pathogenic chlamydia i.e. genes encoding the type III secretion system and polymorphic membrane proteins are regulated differently by the strains.
