Project description:Analysis of TE-1 cells following knockdown of EI24 and control vector. Etoposide induced 2.4 kb transcript (EI24), also known as p53-induced gene 8 (PIG8), is located on human chromosome 11q23. Serving as a p53 responsive pro-apoptotic factor, EI24 plays a pivotal role in inhibiting cell growth and activating autophagy and is associated with drug resistance. Results provide insight into the role of EI24 in cell proliferation and drug resistance of ESCC cells.
Project description:Analysis of EC109 cells following overexpression of EI24 and control vector. Etoposide induced 2.4 kb transcript (EI24), also known as p53-induced gene 8 (PIG8), is located on human chromosome 11q23. Serving as a p53 responsive pro-apoptotic factor, EI24 plays a pivotal role in inhibiting cell growth and activating autophagy and is associated with drug resistance. Results provide insight into the role of EI24 in cell proliferation and drug resistance of ESCC cells.
Project description:To investigate the role of YY1 in ESCC, we established shYY1 ESCC cell lines in which target gene has been knocked down by shRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of shNC and shYY1 cells.
Project description:To understand the difference of protein expression between paired esophageal squamous cell carcinoma (ESCC) and adjacent normal tissues, we collected 10 paired ESCC and normal tissues from surgical resected specimems for high-throughput proteomic experiments. From comparative analysis, the dysregulated signaling pathways in ESCC could be uncovered.
Project description:To discover ESCC related proteins, we used SWATH to quantify the protein abundance between ESCC and adjacent tissues. Briefly, we pooled 10 ESCCtissues and their corresponding adjacent tissues for SWATH acquisition with three replicates.Three DDA repeats were also acquired with the pooled 10-paired ESCC tissue.The trypsin digested peptide mixture was analyzed by AB SCIEX 5600 (AB SCIEX).The database searching procedure was achieved using ProteinPilot v4.5 (AB Sciex). The database is IPI_homo_sapiens_V3.87.
Project description:This dataset contains raw ChIP-seq data from esophageal squamous cell carcinoma (ESCC) cells overexpressing TFAP2β or its LLPS-deficient mutants (ΔIDR3 or Mut_IDR3). The experiment aims to explore the chromatin binding profiles of TFAP2β and its phase separation-deficient mutants to uncover the role of TFAP2β's LLPS activity in regulating gene expression in ESCC. Data were collected from the genomic binding of TFAP2β and its LLPS-deficient mutants, providing insights into the regulatory networks controlled by TFAP2β in cancer progression.
