Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing of LSK EpCAM+ and EpCAM- of Zeb1-/-

ABSTRACT: Next Generation Sequencing of LSK EpCAM+ and EpCAM- of Zeb1-/-

PROVIDER: PRJNA646807 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12244911_1.fastq.gz	Fastqsanger.gz
	SRR12244911_2.fastq.gz	Fastqsanger.gz
	SRR12244912_1.fastq.gz	Fastqsanger.gz
	SRR12244912_2.fastq.gz	Fastqsanger.gz
	SRR12244913_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Next Generation Sequencing of LSK EpCAM+ and EpCAM- of Zeb1-/-

Project description:Zeb1, a zinc finger E-box binding homeobox epithelial-mesenchymal (EMT) transcription factor, confers properties of ‘stemness’, such as self-renewal, in cancer. Yet little is known about the function of Zeb1 in adult stem cells. Here, we used the hematopoietic system, as a well-established paradigm of stem cell biology, to evaluate Zeb1 mediated regulation of adult stem cells. We employed a conditional genetic approach using the Mx1-Cre system to specifically knockout (KO) Zeb1 in adult hematopoietic stem cells (HSCs) and their downstream progeny. Acute genetic deletion of Zeb1 led to rapid onset thymic atrophy and apoptosis driven loss of thymocytes and T cells. A profound cell-autonomous self-renewal defect and multi-lineage differentiation block was observed in Zeb1 KO HSCs. Loss of Zeb1 in HSCs activated transcriptional programs of deregulated HSC maintenance and multi-lineage differentiation genes, and of cell polarity, consisting of cytoskeleton, lipid metabolism/lipid membrane and cell adhesion related genes. Notably, Epithelial cell adhesion molecule (EpCAM) expression was prodigiously upregulated in Zeb1 KO HSCs, which correlated with their enhanced cell survival capacity and diminished differentiation in transplantation. Thus, Zeb1 acts as a crucial transcriptional regulator in hematopoiesis, co-ordinating HSC self-renewal and multi-lineage differentiation fates, in part, via EpCAM repression.

2020-10-01 | GSE154615 | GEO

Next Generation Sequencing of Wild Type and Zeb1-/- HSCs

2020-07-02 | GSE153663 | GEO

Next Generation Sequencing of Wild Type and Zeb1-/- CLP

2020-07-02 | GSE153662 | GEO

Next Generation Sequencing of Wild Type and Zeb1-/- CLP

Project description:Next Generation Sequencing of Wild Type and Zeb1-/- CLP

| PRJNA643569 | ENA

Next Generation Sequencing of Wild Type and Zeb1-/- HSCs

Project description:Next Generation Sequencing of Wild Type and Zeb1-/- HSCs

| PRJNA643571 | ENA

Next Generation Sequencing of Wild Type and Zeb1-/- CLP and HSCs

Project description:Next Generation Sequencing of Wild Type and Zeb1-/- CLP and HSCs

| PRJNA643566 | ENA

Next Generation Sequencing Analysis of Wild Type and Chd8-/- LSK in Transcriptomes, Chromatin accessibility and chromatin state mapping

Project description:Next-generation sequencing (NGS) has been used for study the transcriptomic and genomic change after Chd8 dletion. The goals of this study are to compare NGS-derived LSK transcriptome profiling (RNA-seq), ATAC and H3K4me3 and H3K27me3 Cut&Run data to find downstream targets of CHD8 and its impact on chromatin state.

2021-07-28 | GSE161613 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of db/m and db/db bone marrow derived LSK (Lineage negative Sca1-ckit positive cells) Transcriptomes II

Project description:The Lin-Sca1+c-Kit+ (LSK) fraction of the bone marrow (BM) comprises multipotent hematopoietic stem cells (HSCs), which are vital to tissue homeostasis and vascular repair. While diabetes affects HSC homeostasis overall, the molecular signature of transcriptome under the conditions of long-standing type 2 diabetes (T2D;>6 months) remains unexplored. We employed the strength of Next generation sequencing to to study the differential pattern of LSK transcriptome under chronic diabetes.

2024-07-30 | GSE261502 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of db/m and db/db bone marrow derived LSK (Lineage negative Sca1-ckit positive cells) Transcriptomes I

2024-07-30 | GSE261501 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of ZEB1-Regulated Transcriptomes

Project description:The goal of this study is to analyze the transcripts regulated by ZEB1.

2017-08-10 | GSE102337 | GEO