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Analysis of m6A methylome in CHAPIR overexpressed heart [MeRIP]

ABSTRACT: Analysis of m6A methylome in CHAPIR overexpressed heart [MeRIP]

PROVIDER: PRJNA647217 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12265411.fastq.gz	Fastqsanger.gz
	SRR12265412.fastq.gz	Fastqsanger.gz
	SRR12265413.fastq.gz	Fastqsanger.gz
	SRR12265414.fastq.gz	Fastqsanger.gz

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Analysis of m6A methylome in CHAPIR overexpressed heart [MeRIP]

Project description:To elucidate the molecular mechanism by which cardiac-hypertrophy-associated piRNA (CHAPIR) regulates m6A modification, we performed m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) in control or CHAPIR-overexpressing mice heart. The sequential analysis of m6A peaks showed that RGACH motif was highly enriched within m6A sites in heart and that is aligning with the classical consensus sequence of mammals ‘RGACH’, where ‘R’ indicates purine (A/G) and ‘H’ indicates non-guanine base (A/C/U). In CHAPIR treated heart, m6A mostly occurred in mRNAs (89.5%) and only about 10.5% were identified in non-coding RNAs. The majority of mRNAs contain one or two m6A peaks (89.2%) and 10.8% mRNA contain >2 m6A peaks . M6A peaks were predominantly distributed in coding sequences (CDSs), 3’ untranslated regions (3’UTRs) and near stop codon.

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