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Dissecting the yeast heat shock response by CRISPRi/a

ABSTRACT: Dissecting the yeast heat shock response by CRISPRi/a

PROVIDER: PRJNA649790 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12355400_1.fastq.gz	Fastqsanger.gz
	SRR12355400_2.fastq.gz	Fastqsanger.gz
	SRR12355401_1.fastq.gz	Fastqsanger.gz
	SRR12355401_2.fastq.gz	Fastqsanger.gz
	SRR12355402_1.fastq.gz	Fastqsanger.gz

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Dissecting the yeast heat shock response by CRISPRi/a

Project description:CRISPRi screens on the repression of 129 protein kinases and 161 transcription factors in S. cerevisiae. We quantify perturbation effects on cellular fitness at 23, 30 and 38°C, expression of the SSA1 Hsp70 chaperone (as proxy for heat shock response activity) and thermotolerance. The integration of these phenotypes allowed us to identify core signaling pathways of the HSR and their contributions to temperature-associated growth and heat resistance.

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Project description:Life is resilient because living systems are able to respond to elevated temperatures with an ancient gene expression program called the heat shock response (HSR). Our global analysis revealed a modular HSR dependent on the severity of the stress in yeast. Interestingly, at all temperatures analyzed, the transcription of hundreds of genes is upregulated among them the molecular chaperones, which protect proteins from aggregation. However, for approximately 90% of the regulated genes, the function under stress remained enigmatic. Surprisingly, the majority of these upregulated genes is translated but only for a small fraction this results in raised proteins levels. In this context, increased translation is required to counter-balance elevated protein turnover at elevated temperatures. This anaplerotic reaction together with the molecular chaperone system allows yeast to buffer proteotoxic stress. When the capacity of this system is exhausted at extreme temperatures, translation is stopped via phase transition and growth stops.

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