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Transcriptomic analysis of mouse spermatogonia and human spermatogenesis

ABSTRACT: Transcriptomic analysis of mouse spermatogonia and human spermatogenesis

PROVIDER: PRJNA649962 | ENA |

REPOSITORIES: ENA

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Transcriptomic analysis of mouse spermatogonia

Project description:Transcriptomic analysis of mouse spermatogonia

| PRJNA649964 | ENA

Transcriptomic analysis of human spermatogenesis

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Transcriptomic analysis of mouse spermatogonia

Project description:Microarray analysis was performed in order to detail the gene expression profiles in murine b-2M-SPa-6+c-kit-undifferentiated and b-2M-SPa-6+c-kit+ differentiating spermatogonia. These data were used to compare human and mouse transcriptomes of undifferentiated spermatogonia.

2020-08-01 | GSE155507 | GEO

Deletion of Mgat2 in Spermatogonia Blocks Spermatogenesis

Project description:Deletion of Mgat2 in Spermatogonia Blocks Spermatogenesis

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Regulatory mechanisms on the early process of mouse spermatogenesis investigated by digital gene expression tag profiling analysis

Project description:A detailed understanding of the gene regulation mechanisms in premeiosis cells is germane to understanding normal spermatogenesis. The genome-wide expression profiles of type B spermatogonia and primary spermatocytes in mouse were investigated using Solexa/Illumina’s digital gene expression (DGE) system, a tag based high-throughput transcriptome sequencing method, and the developmental process of early spermatogenesis was analyzed systematically. The results suggested that the expression pattern of mouse type B spermatogonia and primary spermatocytes was changed dramatically, especially the genes related to junction assembly, regulation of actin cytoskeleton and pluripotency. The change of the expression levels of these pathways’ main components may be affected by corresponding miRNAs through post-transcriptional regulation. The analysis results will benefit for better understanding the molecular mechanism of early spermatogenesis process. Using DGE technology to examine gene expression pattern's change between mouse type B spermatogonia and primary spermatocytes cell types.

2015-06-20 | E-GEOD-38845 | biostudies-arrayexpress

Deletion of Mgat2 in Spermatogonia Blocks Spermatogenesis

Project description:Identifying factors required for spermatogenesis is important for understanding mechanisms of male fertility. Inactivation of the Mgat1 or Man2a2 gene leads to a block in spermatogenesis causing infertility in male mice. In both cases multi-nucleated germ cells (MNC) are formed and there are no sperm in the epididymis. MGAT1 GlcNAc-transferase initiates complex N-glycan synthesis and MAN2A2 mannosidase generates the substrate for MGAT2 GlcNAc-transferase which transfers GlcNAc to form a biantennary complex N-glycan. Deletion of Mgat2 can leave its substrate, a hybrid N-glycan terminating in a single GlcNAc, to accumulate or the single GlcNAc may be extended to with Gal and GlcNAc to form polylactosamine. In this paper we show that conditional deletion of Mgat2 in spermatogonia via Stra8-iCre caused a block in spermatogenesis prior to the formation of round spermatids. This phenotype differs from deletion of Mgat1 by Stra8-iCre which generates MNC of round and elongated spermatids. In addition, RNA-seq analysis of germ cells prepared at 15 days after birth revealed a unique transcriptomic landscape in Mgat2[-/-] germ cells. Bioinformatic analyses predicted potential roles for ERK and AKT activities which were validated by western analyses. By contrast, Mgat1[-/-] germ cells have reduced ERK activity and unchanged AKT activity. Therefore, the defective spermatogenesis observed following removal of MGAT1 or MGAT2 must arise from the different immature N-glycans that accumulate rather than from the loss of complex N-glycans which is common to both.

2024-08-30 | GSE264025 | GEO

Regulatory mechanisms on the early process of mouse spermatogenesis investigated by digital gene expression tag profiling analysis

2015-06-20 | GSE38845 | GEO

Proteomic analysis of the effects of DDX20 deletion in spermatogonia

Project description:To investigate the role of DDX20 in spermatogenesis, we generated Ddx20 flox/flox Ddx4-Cre mice to make a germ cell-specific Ddx20 knockout, and isolated spermatogonia from four-day-old mouse testes by THY1 magnetic beads. We then performed proteomic analysis using protein lysates obtained from THY1 + spermatogonia.

2024-04-24 | PXD038170 |

Transcriptomic analysis of mouse spermatogonia and human spermatogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-08-01 | GSE155509 | GEO

GFRα1+, NGN3+ and KIT+ spermatogonia of adult mouse testes

Project description:In adult mouse testis, undifferentiated spermatogonia include GFRα1+ and NGN3+ populations. The former mainly act as self-renewing stem cells that support steady-state spermatogenesis. Whilst, the latter are primed for differentiation, but retain the potential of self-renewal and contribute to regeneration and post-transplantation colony formation (Nakagawa et al. science .2010; Hara et al. cell stem cell. 2014). And, NGN3+ cells can differentiate to KIT+ cells, which are also mentioned differentiated spermatogonia, by retinoic acid signal. The gene expression profiles of GFRα1+, NGN3+ and KIT+ spermatogonia, as well as the whole testis samples, were investigated here by microarray (Ikami et al. development. 2015).

2015-12-02 | GSE75532 | GEO

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