ABSTRACT: RNA Sequencing facilitates quantitative Analysis of differently expressed genes with GA or SKN treatment in rheumatoid fibroblast-like synoviocytes
Project description:RNA Sequencing facilitates quantitative Analysis of differently expressed genes with GA or SKN treatment in rheumatoid fibroblast-like synoviocytes
Project description:RNA Sequencing facilitates quantitative analysis of differently expressed genes with GA or SKN treatment in rheumatoid fibroblast-like synoviocytes
Project description:RNA Sequencing facilitates quantitative Analysis of differently expressed genes with negative control (NC) and siLINK-A treatment in rheumatoid fibroblast-like synoviocytes
Project description:RNA Sequencing facilitates quantitative analysis of differently expressed genes with negative control (NC), siHASML, and siHuR treatment in rheumatoid fibroblast-like synoviocytes
Project description:Fibroblast-like synoviocytes (FLSs) are critical for synovial aggressiveness and joint destruction in rheumatoid arthritis (RA).The role and expression patterns of long noncoding RNAs (lncRNAs) in RA are largely unknown. We performed lncRNA and mRNA microarrays to identify differentially expressed lncRNAs and mRNAs in fibroblast-like synoviocytes from rheumatoid arthritis patients compared with fibroblast-like synoviocytes from trauma patients.
Project description:LncRNA and mRNA microarrays were performed to identify differentially expressed lncRNAs and mRNAs in fibroblast-like synoviocytes from rheumatoid arthritis patients compared with fibroblast-like synoviocytes from trauma patients. Fibroblast-like synoviocytes were isolated from synovial tissues. LncRNA and mRNA microarrays were performed using fibroblast-like synoviocytes at passage 3.
Project description:LncRNA and mRNA microarrays were performed to identify differentially expressed lncRNAs and mRNAs in fibroblast-like synoviocytes from rheumatoid arthritis patients compared with fibroblast-like synoviocytes from trauma patients.
Project description:RNA Sequencing facilitates quantitative Analysis of differently expressed genes with negative control (NC) and siLINK-A treatment in rheumatoid fibroblast-like synoviocytes [RNA-seq]
Project description:To explore downstream gene expression profile of PKM2,we evaluated the transcriptome of GA- or SKN-treated RA FLSs compared to untreated control using RNA sequencing analysis. The data were analyzed for differential expression with a nominal P<0.05. GA Treatment induced downregulation of 46 genes and upregulation of 58 genes compared to DMSO treatment. SKN treatment resulted in downregulation of 14 genes and upregulation of 27 genes compared to DMSO treatment.A Venn diagram showed the overlap of the significantly expressed genes between GA and SKN treatment. We found that in the overlap of both GA and SKN treatment, STAT5A and ARHGAP11A were significantly downregulated, and THAP6 and AHRR were significantly upregulated.
Project description:To characterize transcriptome changes upon ATF6α knockdown by the siRNA in rheumatoid arthritis fibroblast-like synoviocytes (RA FLSs)
