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Transcriptomes of evolved Pseudomonas putida KT2440 on non-native sugars

ABSTRACT: Transcriptomes of evolved Pseudomonas putida KT2440 on non-native sugars

PROVIDER: PRJNA655507 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12396325_1.fastq.gz	Fastqsanger.gz
	SRR12396325_2.fastq.gz	Fastqsanger.gz
	SRR12396326_1.fastq.gz	Fastqsanger.gz
	SRR12396326_2.fastq.gz	Fastqsanger.gz
	SRR12396327_1.fastq.gz	Fastqsanger.gz

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Transcriptomes of evolved Pseudomonas putida KT2440 on non-native sugars

Project description:Efficient utilization of lignocellulosic biomass-derived sugars is essential to improve the economics of biorefinery. While Pseudomonas putida is a promising microbial host, its usage is limited because this strain cannot utilize xylose or galactose as a sole carbon source. To address this issue, we heterologously introduced a xylose utilizing gene (xylD) from Caulobacter crescentus and a galactose operon (galETKM) from E. coli MG1655. To improve the utilization further, we evolved the engineered strains in minimal medium conditions. After the evolution, they acquired better fitnesses on the non-native sugars. To understand transcriptional changes after the evolution, the transcriptomes of few evolved isolates were analyzed.

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