Dissimilatory phosphite oxidizing enrichment metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA655520 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR13787004_1.fastq.gz | Fastqsanger.gz | |||
| SRR13787004_2.fastq.gz | Fastqsanger.gz | |||
| SRR13787005_1.fastq.gz | Fastqsanger.gz | |||
| SRR13787005_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 25 |
Similar Datasets
Project description:Time course study of potato leaflets (cv. Desiree) 3, 6, 11, 24 and 48 hours after phosphite treatment
2014-10-27 | E-MTAB-2243 | biostudies-arrayexpress
Project description:The aim of the present work was to gain insight into the mechanisms of action of potassium phosphite. To achive this, we performed microarray analysis to determine changes in gene expression of potato leaves treated with potassium phosphite for 72 hs and the abundance of KPhi primed genes after Phythophtora infestans infection. We have also analyzed in silico their promoters for common cis elements involved in their regulation.
2019-12-04 | GSE136094 | GEO
Project description:The use of phosphites in disease control management and abiotic stress has proven to be effective. Although the mechanisms underlying their effect remain unclear, it has been postulated that miRNAs could be involved. In order to understand these mechanisms we performed NGS sequencing in potato leaves treated or not with KPhi to identify miRNAs responsive to this biocompatible compound. The aim of the present work is to identify miRNAs that are involved in the regulation of potato defense responses after phosphite treatment.
2019-08-30 | GSE132232 | GEO
Project description:Anaerobic oxidation of diclofenac coupled with dissimilatory iron reduction Raw sequence reads
| PRJNA892742 | ENA
Project description:Phosphorus is an essential limited nutrient for plant growth and modern agricultural productivity. A comprehensive understanding of how plants respond to phosphate starvation is essential to develop more phosphate-use efficient crops. Here we quantified protein-level responses to phosphate (PO4-) and phosphite (PO3-) in Arabidopsis thaliana suspension cells, relative to a phosphate-starved state using label-free proteomics and phosphoproteomics. Phosphite is similarly sensed and taken up by plants as phosphate but cannot be used as a source of phosphorus. Phosphite is hence a useful tool to delineate between non-specific processes related to sensing and transport, and specific responses to phosphorus nutrition. We find that responses to phosphate and phosphite occur mainly at the level of protein phosphorylation, complemented by limited changes in protein abundance, primarily in protein translation, and phosphate transport and scavenging proteins. Phosphorylation changes in proteins involved in core processes such as translation, RNA splicing and kinase signalling are especially important in phosphate starvation. We also find differential phosphorylation in response to phosphate and phosphite in 69 proteins, including splicing factors, translation factors, the PHT1;4 phosphate transporter and the HAT1 histone acetyltransferase—potential phospho-switches sensing changes in phosphorus nutrition. Our study hence illuminates several new aspects of the phosphate-starvation response and identifies important targets for further investigation and crop improvement.
2021-01-04 | PXD018989 | Pride
Project description:Phosphite is the most energetically favorable chemotrophic electron donor known, with a half-cell potential (E o') of -650 mV for the PO4 3-/PO3 3- couple. Since the discovery of microbial dissimilatory phosphite oxidation (DPO) in 2000, the environmental distribution, evolution, and diversity of DPO microorganisms (DPOMs) have remained enigmatic, as only two species have been identified. Here, metagenomic sequencing of phosphite-enriched microbial communities enabled the genome reconstruction and metabolic characterization of 21 additional DPOMs. These DPOMs spanned six classes of bacteria, including the Negativicutes, Desulfotomaculia, Synergistia, Syntrophia, Desulfobacteria, and Desulfomonilia_A Comparing the DPO genes from the genomes of enriched organisms with over 17,000 publicly available metagenomes revealed the global existence of this metabolism in diverse anoxic environments, including wastewaters, sediments, and subsurface aquifers. Despite their newfound environmental and taxonomic diversity, metagenomic analyses suggested that the typical DPOM is a chemolithoautotroph that occupies low-oxygen environments and specializes in phosphite oxidation coupled to CO2 reduction. Phylogenetic analyses indicated that the DPO genes form a highly conserved cluster that likely has ancient origins predating the split of monoderm and diderm bacteria. By coupling microbial cultivation strategies with metagenomics, these studies highlighted the unsampled metabolic versatility latent in microbial communities. We have uncovered the unexpected prevalence, diversity, biochemical specialization, and ancient origins of a unique metabolism central to the redox cycling of phosphorus, a primary nutrient on Earth.
| S-EPMC7980464 | biostudies-literature
Project description:With its capacity for anaerobic methane oxidation and denitrification, the bacterium Methylomirabilis oxyfera plays an important role in natural ecosystems. Its unique physiology can be exploited for more sustainable wastewater treatment technologies. However, operational stability of full-scale bioreactors can experience setbacks due to, for example, bacteriophage blooms. By shaping microbial communities through mortality, horizontal gene transfer, and metabolic reprogramming, bacteriophages are important players in most ecosystems. Here, we analyzed an infected Methylomirabilis sp. bioreactor enrichment culture using (advanced) electron microscopy, viral metagenomics and bioinformatics. Electron micrographs revealed four different viral morphotypes, one of which was observed to infect Methylomirabilis cells. The infected cells contained densely packed ~55 nm icosahedral bacteriophage particles with a putative internal membrane. Various stages of virion assembly were observed. Moreover, during the bacteriophage replication, the host cytoplasmic membrane appeared extremely patchy, which suggests that the bacteriophages may use host bacterial lipids to build their own putative internal membrane. The viral metagenome contained 1.87 million base pairs of assembled viral sequences, from which five putative complete viral genomes were assembled and manually annotated. Using bioinformatics analyses, we could not identify which viral genome belonged to the Methylomirabilis- infecting bacteriophage, in part because the obtained viral genome sequences were novel and unique to this reactor system. Taken together these results show that new bacteriophages can be detected in anaerobic cultivation systems and that the effect of bacteriophages on the microbial community in these systems is a topic for further study.
| S-EPMC5099504 | biostudies-literature
Project description:The presence of a copper-containing dissimilatory nitrite reductase gene (nirK) was discovered in several isolates of beta-subdivision ammonia-oxidizing bacteria using PCR and DNA sequencing. PCR primers Cunir3 and Cunir4 were designed based on published nirK sequences from denitrifying bacteria and used to amplify a 540-bp fragment of the nirK gene from Nitrosomonas marina and five additional isolates of ammonia-oxidizing bacteria. Amplification products of the expected size were cloned and sequenced. Alignment of the nucleic acid and deduced amino acid (AA) sequences shows significant similarity (62 to 75% DNA, 58 to 76% AA) between nitrite reductases present in these nitrifiers and the copper-containing nitrite reductase found in classic heterotrophic denitrifiers. While the presence of a nitrite reductase in Nitrosomonas europaea is known from early biochemical work, preliminary sequence data from its genome indicate a rather low similarity to the denitrifier nirKs. Phylogenetic analysis of the partial nitrifier nirK sequences indicates that the topology of the nirK tree corresponds to the 16S rRNA and amoA trees. While the role of nitrite reduction in the metabolism of nitrifying bacteria is still uncertain, these data show that the nirK gene is present in closely related nitrifying isolates from many oceanographic regions and suggest that nirK sequences retrieved from the environment may include sequences from ammonia-oxidizing bacteria.
| S-EPMC92858 | biostudies-literature
Project description:Here, we report a new metagenome-assembled genome (MAG) from a marine Rhizobiaceae species. The MnEN-MB40S genome was assembled from a manganese-oxidizing enrichment culture metagenome. A 4.1-Mb MAG comprising 26 contigs, with a GC content of 60.0%, was obtained. This MAG contributes to the genomic information regarding the family Rhizobiaceae.
| S-EPMC9476993 | biostudies-literature
Project description:Desulfotignum phosphitoxidans is a strictly anaerobic, Gram-negative bacterium that utilizes phosphite as the sole electron source for homoacetogenic CO2 reduction or sulfate reduction. A genomic library of D. phosphitoxidans, constructed using the fosmid vector pJK050, was screened for clones harboring the genes involved in phosphite oxidation via PCR using primers developed based on the amino acid sequences of phosphite-induced proteins. Sequence analysis of two positive clones revealed a putative operon of seven genes predicted to be involved in phosphite oxidation. Four of these genes (ptxD-ptdFCG) were cloned and heterologously expressed in Desulfotignum balticum, a related strain that cannot use phosphite as either an electron donor or as a phosphorus source. The ptxD-ptdFCG gene cluster was sufficient to confer phosphite uptake and oxidation ability to the D. balticum host strain but did not allow use of phosphite as an electron donor for chemolithotrophic growth. Phosphite oxidation activity was measured in cell extracts of D. balticum transconjugants, suggesting that all genes required for phosphite oxidation were cloned. Genes of the phosphite gene cluster were assigned putative functions on the basis of sequence analysis and enzyme assays.
| S-EPMC2944520 | biostudies-literature