Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single Cell RNA Sequencing of fetal mouse neurosphere cultures.

ABSTRACT: Single Cell RNA Sequencing of fetal mouse neurosphere cultures.

PROVIDER: PRJNA655918 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12413776.fastq.gz	Fastqsanger.gz
	SRR12413777.fastq.gz	Fastqsanger.gz
	SRR12413778.fastq.gz	Fastqsanger.gz
	SRR12413779.fastq.gz	Fastqsanger.gz
	SRR12413780.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Single Cell RNA Sequencing of fetal mouse neurosphere cultures.

Project description:We performed single-cell RNA sequencing on neurosphere cultures derived from the dorsal telencephalic vesicles of sex segregated gestational day (GD) 12.5 C57BL/6J mouse fetuses.

2021-02-10 | GSE155880 | GEO

Single-cell transcriptomic profiling of mouse enteric neurons and enteric mesenchymal cells in enteric neurosphere cultures.

Project description:This study was undertaken to define the molecular subtypes of cells within enteric neurospheres. Methods: We performed single-cell RNA sequencing on 3-dimensional neurosphere cultures derived from the small intestines of Plp1::GFP mice. These data show profiling results from the PLP1::GFP negative cell fraction ancillary to the positive fraction in series GSE184981. Results: We identify populations of enteric neurons and fibroblasts in the PLP1::GFP negative cell fraction of enteric neurospheres.

2024-07-16 | GSE264155 | GEO

Single-cell transcriptomic profiling of mouse enteric neurons and enteric mesenchymal cells in enteric neurosphere cultures.

Project description:Single-cell transcriptomic profiling of mouse enteric neurons and enteric mesenchymal cells in enteric neurosphere cultures.

| PRJNA1101063 | ENA

Comparison between mouse ES/iPS derived neurosphere and mouse primary culture of neurospheres obtained from fetal mouse ganglionic eminence

Project description:Recent reports have emphasized the pitfalls of iPSC technology including the potential for immunogenicity of transplanted cells. It is serious safety-related concern for iPSC-based cell therapy. However, preclinical data supporting the safety and efficacy of iPSCs are also accumulating. To address the concern of immunogenicity of ESCs/iPSCs or ESCs/iPSCs-derived neurospheres, global gene expression profiles were compared between undifferentiated mouse ESCs (EB3 line), mouse iPSCs (38C2 line), and ESC/iPSC-derived neurosphere and mouse primary culture of neurosphere obtained from fetal mouse ganglionic eminence. Mouse adult sklin fibroblast was used as a control. We used affymetrix microarrays to compare the global gene expression of neurospheres prepared several origins. Keywords: Expression profiling by array

2012-03-01 | GSE31725 | GEO

Mouse Fetal Cortical-derived Neurosphere cultures transfected with null control or miRNA-153 mimetic

Project description:The expression of the mciroRNA, miR-153 is significantly altered in neural stem cells (NSCs) following exposure to the teratogens, alcohol and nicotine (PMIDs:22458409, 17687032). To better understand how miR-153 may mediate teratogenesis, we first needed to identify miR153 targets in fetal NSCs. Gestational day 12.5 fetal mouse cortical neural epithelium was isolated and grown as neurospheres in defined medium for studies. We overexpressed miR-153 in fetal NSCs for 24 hours in a transient transfection assay, and profiled mRNA expression using a microarray platform (codelink array) to identify potential target genes. Over expression of pre-miR-153 resulted in a ~32-fold induction of miR-153 compared to the transfection control. Transcript profiles were assessed from RNA samples (6 independent replicates in each condition) hybridized to codelink microarrays according to manufacturer's protocols. Data analysis showed that miR-153 overexpression resulted in statistically significant down-regulation of 102 genes (at a false discovery rate α=0.1, of a total of 860 transcripts down-regulated by ≥1.3-fold). These data suggested the role of this miRNA in moderately controlling expression hundreds of gene transcripts. These regulated genes may be important in controlling neural stem cell differentiation and downstream signaling pathways. Six independent replicates were performed for control and treatment groups, and twelve independent single channel intensity values were uploaded after normalization for analysis independent single channel intensity values were uploaded after normalization

2014-08-01 | E-GEOD-49684 | biostudies-arrayexpress

Comparison between mouse ES/iPS derived neurosphere and mouse primary culture of neurospheres obtained from fetal mouse ganglionic eminence

2012-03-01 | E-GEOD-31725 | biostudies-arrayexpress

Mouse Fetal Cortical-derived Neurosphere cultures transfected with null control or miRNA-153 mimetic

2014-08-01 | GSE49684 | GEO

scRNA neurosphere model

Project description:scRNA neurosphere model

| PRJEB62840 | ENA

Study on plasma proteomics in single intrauterine fetal death

Project description:Study on plasma proteomics in single intrauterine fetal death

2021-09-07 | PXD040710 |

Transcription profiling of murine neurosphere cultures carrying a Bcl2 transgene and treated with histone deacetylase inhibitor Trichostatin A and demethylating agent AzaC to study DNA methylation and histone deacetylation in neural stem cells.

Project description:Neurosphere cultures were established from individually isolated E 14.5 forebrains of mouse embryos that carry a bcl2 transgene. Single-cell suspensions were prepared, seeded at 1x105 cells/ml and treated for two days with 150 nM Trichostatin A (TSA; histone deacetylase inhibitor), 500 nM 5-Aza-2-deoxycytidine (AzaC; demethylating agent) or both compounds, or left untreated. Two independent experiments were performed.

2007-12-19 | E-GEOD-587 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data