Project description:We performed single-cell RNA sequencing on neurosphere cultures derived from the dorsal telencephalic vesicles of sex segregated gestational day (GD) 12.5 C57BL/6J mouse fetuses.
Project description:Understanding the molecular bases of neurological and psychiatric conditions is hampered by a lack of suitable patient-derived cellular models. The olfactory mucosa is a continually regenerating neural tissue that contains multipotent neural stem cells which are accessible and expandable in vitro as neurospheres. Here we demonstrate disease-specific differences in neurosphere-derived cell lines from patients with two unrelated neurological conditions. Lines derived from donors with schizophrenia exhibited significant dysregulation of neurodevelopmental pathways whereas those obtained from donors with Parkinson's disease demonstrated significant dysgregulation in mitochondrial function, oxidative stress and xenobiotic metabolism. Our model supports the hypothesis that diseases with multiple genetic and environmental risk factors are revealed in convergent cellular and molecular pathways in olfactory neurosphere-derived cells taken from patient cohorts. These patient-derived cells provide informative models that provide insights into neurobiological correlates of neurological and psychiatric disorders that may be useful for many neuropsychiatric/neurodegenerative conditions and for drug discovery
Project description:We studied the membrane protein compositions of Streptococcus pneumoniae WT and scRNA mutant strains, with or without the CSP1 induction into competence state.
Project description:To determine fluoxetine-induced transcriptional signatures in GBM cancer cells, we performed RNA-sequencing analysis of three GBM patient-derived neurosphere cancer cell lines after fluoxetine or DMSO treatment. Genes with differential expression and associated transcriptional signatures were analyzed and identified in three cancer cell lines.
Project description:Nasal biopsies were collected from control and A-T patients prior to establishing olfactory neurosphere derived (ONS) cells. These cells were phenotyped using stem cell markers, a series of A-T cellular characteristics determined and cells differentiated into neuronal cells.
Project description:Recent reports have emphasized the pitfalls of iPSC technology including the potential for immunogenicity of transplanted cells. It is serious safety-related concern for iPSC-based cell therapy. However, preclinical data supporting the safety and efficacy of iPSCs are also accumulating. To address the concern of immunogenicity of ESCs/iPSCs or ESCs/iPSCs-derived neurospheres, global gene expression profiles were compared between undifferentiated mouse ESCs (EB3 line), mouse iPSCs (38C2 line), and ESC/iPSC-derived neurosphere and mouse primary culture of neurosphere obtained from fetal mouse ganglionic eminence. Mouse adult sklin fibroblast was used as a control. We used affymetrix microarrays to compare the global gene expression of neurospheres prepared several origins. Keywords: Expression profiling by array
