Project description:Co-ip assay was used to conduct protein interaction between ARP1 wild-type cells and ARP1 CBL overexpressed cells. After adhesive strips were cut, samples were sent for mass spectrometry sequencing to search for downstream proteins
Project description:Transcriptome sequencing was used to analyze changes in related signaling pathways and gene expression in ARP1 cells when VCP was knocked down.
Project description:To investigated the mechanism of HNRNPA2B1 in MM tumorigenesis, performed m6A IP seq analysis (MeRIP-Seq) to identify the expression changes in stable HNRNPA2B1-knockdown MM cells.60 genes m6A with over 2-fold expression change in shHNRNPA2B compared with control in both ARP1 and H929 cells.44 differentially expressed genes (P<0.05) in both m6A and transcription in shHNRNPA2B compared with control in ARP1 cell.
Project description:To explore the function of PNPO, we constructed ARP1 and H929 pTSB-EV/PNPO-OE cell lines via lentivirus packaging. We found different genes change between EV and OE cells.
Project description:To determine what signaling pathways are affected by I3MO in MM cells, 5 MM cell lines including ANBL6, ANBL6 BR, ARP1, RPMI-8226, and U266, were cultured with DMSO or 5/10µM I3MO for 48 h. Total RNAs of 2 x 10^6 cells for each sample were extracted using the RNeasy Mini Kit (Qiagen). The RNA yield was determined by Nanodrop technology (Thermo Fisher Scientific), and quality was verified on the Agilent 2100 bioanalyzer (Agilent Technologies). cDNA libraries were prepared for sequencing using standard Illumina protocols, followed by sequencing on the NextSeq500 Sequencing System (Illumina). We used gene expression profiling data to determine differential expression of genes in MM cells in culture with DMSO or I3MO.