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MiRNAs expression profile in goat peripheral blood mononuclear cells (PBMCs) infected with Nigeria 75/1 vaccine virus

ABSTRACT: miRNAs expression profile in goat peripheral blood mononuclear cells (PBMCs) infected with Nigeria 75/1 vaccine virus

PROVIDER: PRJNA657627 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12463467.fastq.gz	Fastqsanger.gz
	SRR12463468.fastq.gz	Fastqsanger.gz
	SRR12463469.fastq.gz	Fastqsanger.gz
	SRR12463470.fastq.gz	Fastqsanger.gz
	SRR12463471.fastq.gz	Fastqsanger.gz

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miRNAs expression profile in goat peripheral blood mononuclear cells (PBMCs) infected with Nigeria 75/1 vaccine virus

Project description:Peste des petits ruminants virus (PPRV) belongs to the genus Morbillivirus that causes an acute and highly contagious disease in goats and sheep. Virus infection can trigger the change in the cellular microRNA (miRNA) expression profile, which play important post-transcriptional regulatory roles in gene expression and can greatly influence viral replication and pathogenesis. Here, we employed deep sequencing technology to determine cellular miRNAs expression profile in goat peripheral blood mononuclear cells (PBMCs) infected with Nigeria 75/1 vaccine virus, a widely used vaccine strain for mass vaccination programs against Peste des petits ruminants (PPR). Expression analysis demonstrated that PPRV infection can elicit 316 significantly differentially expressed (DE) miRNAs including 103 known and 213 novel miRNAs candidates in infected PBMCs at 24 hours post-infection as compared with mock control. Target prediction and functional analysis of these DEmiRNAs revealed significant enrichment for several signaling pathways including TLR signaling pathways, PI3K-Akt, endocytosis, viral carcinogenesis, and JAK-STAT signaling pathways. This study provides a valuable basis for further investigation on the roles of miRNAs in PPRV replication and pathogenesis.

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Goat Peripheral Blood Mononuclear Cells (PBMCs) responses to infection vary with Peste des Petits Ruminants Virus virulence

Project description:Peste des Petits Ruminants (PPR) is a highly infectious disease caused by a virus of the genus Morbillivirus (PPRV), infecting mainly sheep and goat. Susceptibility of host can vary widely with host breed and virus strain. The mechanisms underlying this variability are not well understood. Here, we carried out the first comparative in vitro study on goat peripheral blood mononuclear cells (PBMCs) infected with PPRV strains of different virulence, vaccine strain (N75/1), low- (IC89), and high-virulent strain (MA08). Goat PBMCs were infected by the different strains and stimulated with Concanavalin A (Con A), and proteome changes of these cells were evaluated during the infection. The level of PPRV replication was assessed first by RT-qPCR quantification of viral N mRNA and by labelling the viral N protein inside the cells with specific antibodies and analysed by flow cytometry. The dynamics of PBMC sub-populations were also evaluated by flow cytometry. Our results showed that viral replication is critical for PPRV inhibitory effect on PBMCs proliferation. Highly virulent MA08 strain reached a higher level of replication in ConA-stimulated PBMCs and induced higher mortality compared to other strains. Low-virulent IC89 strain showed the lower replication level and cell proliferative inhibitory capacities. Differences in immune genes expression levels were assessed using RNAseq analysis and protein expression was compared using mass spectrometry. Analysis of these data provided the transcriptional and proteome landscape of PBMCs infected with these strains. The possible association between the transcriptional and proteome landscape and changes in immune cell subpopulations dynamics was explored.

2021-11-03 | PXD024890 | Pride

Transcriptional profiling of different subsets of PBMCs-CD4+, CD8+, CD14+, CD21+, CD335+ reveals differential response to Sungri/96 PPRV vaccination in Goat and Sheep

Project description:We have used RNA-Seq to examine expression profiles of different subsets of PBMCs-CD4+, CD8+, CD14+, CD21+, CD335+ from Goat and Sheep under sungri/96 PPRV vaccination.

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miR from peripheral blood mononuclear cells' (PBMCs') exosome

Project description:The results (significance versus foldchange) showed 183 up- and 195 downregulated miR in the oxygen-glucose deprivation-PBMCs compared to normoxic PBMCs

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