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Microarray gene expression in RKO cell line under low replication stress

ABSTRACT: Microarray gene expression in RKO cell line under low replication stress

PROVIDER: PRJNA658154 | ENA |

REPOSITORIES: ENA

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Microarray gene expression in RKO cell line under low replication stress

Project description:RKO cells were treated with low doses of aphidicolin (0,2µM) that inhibit replicative DNA polymerases and induce a mild replication stress. Expression data were analysed on control cells (DMSO), 16h of treatment (t0) and after release in complete new culture medium of 13h (N+1) using microarray (affymetrix Clarion S) We used microarray to analyse the impact of low replication stress on gene expression comparing DMSO or aphidicolin-treated cells at the end of the treatment (t0) and in daughter cells released from the replication stress (N+1).

2021-05-19 | GSE156521 | GEO

ATAC-seq_experiment in RKO cell line under low replication stress

Project description:RKO cells were treated with low doses of aphidicolin (0.2µM) that inhibit replicative DNA polymerases and induce a mild replication stress. ATAC-seq data were analysed on control cells (DMSO) and after 16h of treatment with aphidicolin

2021-05-19 | GSE156552 | GEO

ATAC-seq_experiment in RKO cell line under low replication stress

Project description:ATAC-seq_experiment in RKO cell line under low replication stress

| PRJNA658332 | ENA

ChIP-seq of MYST acetyltransferases in RKO cell line

Project description:Genome-wide mapping of MYST acetyltransferase subunit BRPF3 in RKO synchronised cells. Genome-wide mapping of MYST acetyltransferase subunit BRPF3 in RKO synchronised cells.

2016-02-05 | E-GEOD-63722 | biostudies-arrayexpress

ChIP-seq of MYST acetyltransferases in RKO cell line

Project description:Genome-wide mapping of MYST acetyltransferase subunit BRPF3 in RKO synchronised cells.

2016-02-05 | GSE63722 | GEO

Expression data from RKO cells transfected with SPARCL1 and vector control

Project description:Colon cancer cell line RKO grown on Matrigel form a luminal-like structure after the transfection of SPARCL1 gene, which may imply the differentiation of the cells. To indentify genes regulated by SPARCL1, we built two stable cell lines: RKO with pLXSN-SPARCL1 plasmid (RKO-SPARCL1) and RKO with pLXSN plasmid (RKO-pLXSN). Then we cultured these two cell lines on both plastic dish and dish cover with a layer of Matrigel. We used microarrays to undentify global gene expression change in RKO cells after the tranfection of SPARCL1. Four samples including RKO-pLXSN cells cultured on plastic, RKO-pLXSN cells cultured on Matrigel, RKO-SPARCL1 cells cultured on plastic and RKO-SPARCL1 cells cultured on Matrigel were trypsinized and collected for RNA extraction and hybridization on Affymetrix microarray U133plus2.0. Supplementary files: * RLvsRP: up and down regulated genes comparing with RKO-SPARCL1(RL) and RKO-pLXSN(RP) cells cultured on plastic * RLMvsRPM: up and down regulated genes comparing with RKO-SPARCL1(RL) and RKO-pLXSN(RP) cells cultured on Matrigel

2011-09-29 | E-GEOD-31440 | biostudies-arrayexpress

Microarray Experiment for Swarnaprabha rice line in low light stress

Project description:Microarray Experiment for Swarnaprabha rice line in low light stress

| PRJNA485730 | ENA

Expression data from RKO cells transfected with SPARCL1 and vector control

2011-09-30 | GSE31440 | GEO

Replication timing program in 6 human model cell lines

Project description:We present here the characterization of the replication timing program in 6 human cell lines : U2OS, RKO, 293T, HeLa, MRC5 and K562

2016-07-08 | GSE80029 | GEO

Microarray Experiment for Swarnaprabha rice line in low light stress

Project description:To further understand the genomics of low light tolerance, Swarnaprabha rice line was subjected to low light or shade stress. The fully emerged panicles were samples at early grain filling stage and microarray was performed from the spikelets. Expression of 12 gene in different pathway were verified using real-time PCR reactions.

2019-05-14 | GSE118464 | GEO

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