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Regulation of PGC-1alpha function by its C-terminal domain [RNA-seq]

ABSTRACT: Regulation of PGC-1alpha function by its C-terminal domain [RNA-seq]

PROVIDER: PRJNA658470 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12490776_1.fastq.gz	Fastqsanger.gz
	SRR12490776_2.fastq.gz	Fastqsanger.gz
	SRR12490777_1.fastq.gz	Fastqsanger.gz
	SRR12490777_2.fastq.gz	Fastqsanger.gz
	SRR12490778_1.fastq.gz	Fastqsanger.gz

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Regulation of PGC-1alpha function by its C-terminal domain [RNA-seq]

Project description:PGC-1alpha has been proposed to couple gene transcription and RNA processing via SR-rich domains and an RNA recognition motif located at the C-terminal domain (CTD). However, the full extent by which the CTD of PGC-1alpha regulates its function in gene expression and RNA processing remains largely unknown. Here, we used RNA-seq to evaluate the effects of CTD deletion (dCTD) on PGC-1alpha function. We found that deletion of the CTD of PGC-1alpha virtually abolished its effects on transcriptome remodeling and the resulting increase in oxidative capacity in skeletal muscle cells. Moreover, we implemented in vitro affinity purification followed by sequencing (in vitro AP-seq), which revealed more than 700 RNAs bound to the CTD of PGC-1alpha. The majority of these RNAs were transcribed at DNA regulatory elements such as promoters and distal intergenic regions. These results demonstrate that PGC-1alpha function is highly modulated at the CTD via multivalent protein-RNA interactions.

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Regulation of PGC-1alpha function by its C-terminal domain

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Regulation of PGC-1alpha function by its C-terminal domain [in vitro AP-seq]

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Regulation of HSF1-mediated transcriptional programs by PGC-1alpha

Project description:We examined global gene expression patterns in response to PGC-1 expression in cells derived from liver or muscle. As our study revealed regulation of HSF1 by PGC-1alpha, in some experiments we knocked-down HSF1 using siRNAs in addition to inducing PGC-1alpha expression. Cells were grown in 24-well plates and adenoviruses encoding either GFP ("Ad-GFP"), PGC-1alpha ("Ad-PGC-1alpha") or PGC-1beta ("Ad-PGC-1beta") were directly added to the culture medium. For experiments involving siRNA transfections, cells were transfected with the indicated siRNAs 48hr prior to infection with adenoviruses encoding either GFP ("Ad-GFP") or PGC-1alpha ("Ad-PGC-1alpha").

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