Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Regional specialization and fate specification of mesenchymal stromal cells in skeletal development

ABSTRACT: Regional specialization and fate specification of mesenchymal stromal cells in skeletal development

PROVIDER: PRJNA658559 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12492372_1.fastq.gz	Fastqsanger.gz
	SRR12492372_2.fastq.gz	Fastqsanger.gz
	SRR12492373_1.fastq.gz	Fastqsanger.gz
	SRR12492373_2.fastq.gz	Fastqsanger.gz
	SRR12492374_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 26

Similar Datasets

Regional specialization and fate specification of mesenchymal stromal cells in skeletal development [scRNA-seq]

Project description:Regional specialization and fate specification of mesenchymal stromal cells in skeletal development [scRNA-seq]

| PRJNA658566 | ENA

Regional specialization and fate specification of mesenchymal stromal cells in skeletal development [bulk RNA-seq]

Project description:Regional specialization and fate specification of mesenchymal stromal cells in skeletal development [bulk RNA-seq]

| PRJNA658565 | ENA

Regional specialization and fate specification of bone stromal cells in skeletal development.

Project description:Bone stroma contributes to the regulation of osteogenesis and hematopoiesis but also to fracture healing and disease processes. Mesenchymal stromal cells from bone (BMSCs) represent a heterogenous mixture of different subpopulations with distinct molecular and functional properties. The lineage relationship between BMSC subsets and their regulation by intrinsic and extrinsic factors are not well understood. Here, we show with mouse genetics, ex vivo cell differentiation assays, and transcriptional profiling that BMSCs from metaphysis (mpMSCs) and diaphysis (dpMSCs) are fundamentally distinct. Fate-tracking experiments and single-cell RNA sequencing indicate that bone-forming osteoblast lineage cells and dpMSCs, including leptin receptor-positive (LepR+) reticular cells in bone marrow, emerge from mpMSCs in the postnatal metaphysis. Finally, we show that BMSC fate is controlled by platelet-derived growth factor receptor β (PDGFRβ) signaling and the transcription factor Jun-B. The sum of our findings improves our understanding of BMSC development, lineage relationships, and differentiation.

| S-EPMC8293626 | biostudies-literature

Regional specialization and fate specification of mesenchymal stromal cells in skeletal development

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-07-27 | GSE156636 | GEO

Regional specialization and fate specification of mesenchymal stromal cells in skeletal development [scRNA-seq]

Project description:To identify cellular heterogeneity and molecular signatures of bone marrow stromal (BMSCs) cells, we performed scRNA-seq of non-hematopoietic stromal cells from 3-week-old mice. In addition, to gain further insight into MSC differentiation in vivo, Pdgfrb-CreERT2 Rosa26-mTmG (Pdgfrb-Cre R26-mT/mG) double transgenic mice were treated with tamoxifen at P1-3 and sacrificed at P21 for the isolation of GFP+ cells from long bone followed by scRNA-seq analysis.

2021-07-27 | GSE156635 | GEO

Regional specialization and fate specification of mesenchymal stromal cells in skeletal development [bulk RNA-seq]

Project description:To gain insight into the molecular signatures and heterogenity of bone mesenchymal stromal cells population between metaphysis (mpMSC) and diaphysis (dpMSC). mpMSCs and dpMSCs were sorted from three weeks old Hey1-GFP metaphysis and Pdgfra-EGFP BM using a flow cytometer for bulk-RNA sequencing analysis.

2021-07-27 | GSE156634 | GEO

Molecular programs of regional specification and neural stem cell fate progression in developing macaque telencephalon

Project description:During early telencephalic development, intricate processes of regional patterning and neural stem cell (NSC) fate specification take place. However, our understanding of these processes in primates, including both conserved and species-specific features, remains limited. Here, we profiled 761,529 single-cell transcriptomes from multiple regions of the prenatal macaque telencephalon. We deciphered the molecular programs of the early organizing centers and their cross-talk with NSCs, revealing primatebiased galanin-like peptide (GALP) signaling in the antero-ventral telencephalon. Regional transcriptomic variations were observed along the fronto-temporal axis during early stages of neocortical NSC progression and in neurons and astrocytes. Additionally, we found that genes associated with neuropsychiatric disorders and brain cancer risk might play critical roles in the early telencephalic organizers and during NSC progression.

2023-10-13 | GSE226451 | GEO

Molecular programs of regional specification and neural stem cell fate progression in developing macaque telencephalon

Project description:Molecular programs of regional specification and neural stem cell fate progression in developing macaque telencephalon

| PRJNA940362 | ENA

Aging of hematopoietic stem cells is driven by regional specialization of marrow macrophages

Project description:Aging of hematopoietic stem cells is driven by regional specialization of marrow macrophages

| PRJNA393409 | ENA

Metabolite analysis of regional neural stem/progenitor cells

Project description:The developing mammalian brain generates a variety of Neural Progenitor Cells (NPCs). Primary NPCs throughout the neuraxis are derived from the ventricular zone. Intermediate progenitor cells (IPCs) are produced uniquely in the telencephalon and contribute extensively to the neurons that comprise the cerebral cortex and basal ganglia. It is known that the fate of the diverse NPC populations is determined by the interplay of transcription factors and regulation by regional humoral cues. However, despite our recent appreciation that nutrient-regulated intracellular metabolic milieu (pO2, energy, and redox state) significantly influence cell fate, an unexplored area is whether NPCs have intrinsic metabolic identity, and if so, the mechanism by which molecular metabolism contributes to brain development.Little is known however, if intrinsic differences in cellular metabolism of regional NPCs make certain NPCs susceptible while others resistant to genetic and environmental insults. We conjectured that regional (fore-and hindbrain) NPCs are metabolically distinct.

2017-06-19 | ST000767 | MetabolomicsWorkbench

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data