Project description:Here, we present the first plastome of Ludwigia octovalvis (Onagraceae, Myrtales) as well as the first plastome in the subfamily Ludwigioideae. This genome is notable for its contracted inverted repeat regions and an expanded small single-copy region compared to other species in the orders Myrtales and Geraniales.
Project description:Current antiobesity and antidiabetic tools have been insufficient to curb these diseases and frequently cause side effects; therefore, new pancreatic lipase and α-glucosidase inhibitors could be excellent aids for the prevention and treatment of these diseases. The aim of this study was to identify, quantify, and characterize the chemical compounds with the highest degree of inhibitory activity of these enzymes, contained in a Ludwigia octovalvis hydroalcoholic extract. Chemical purification was performed by liquid-liquid separation and column chromatography. Inhibitory activities were measured in vitro, employing acarbose, orlistat, and a Camellia sinensis hydroalcoholic extract as references. For structural elucidation, Nuclear Magnetic Resonance was carried out, and High Performance Liquid Chromatography was used to quantify the compounds. For α-glucosidases, L. octovalvis hydroalcoholic extract and its ethyl acetate fraction showed half-maximal Inhibitory Concentration (IC50) values of 700 and 250 μg/mL, for lipase, 480 and 718 μg/mL, while C. sinensis showed 260 and 587 μg/mL. The most active compounds were identified as ethyl gallate (1, IC50 832 μM) and gallic acid (2, IC50 969 μM); both displayed competitive inhibition of α-glucosidases and isoorientin (3, IC50 201 μM), which displayed uncompetitive inhibition of lipase. These data could be useful in the development of a novel phytopharmaceutical drug.
Project description:In 1953, Hara provided new combinations for many sectional and species names when he combined Jussiaea L. with Ludwigia L., and at the time, Ludwigiasect.Oligospermum (Micheli) H.Hara was the correct name for one well-defined section. However, subsequent changes to/clarifications of the botanical code have necessitated a change for that name in that now an autonym is treated as having priority over the name or names of the same date and rank that established it. Since Hara's combination was based on Jussiaeasect.Oligospermum Micheli, the correct name for this section is Ludwigiasect.Jussiaea (L.) Hoch, W.L.Wagner, & P.H.Raven.
Project description:We investigated the anti-aging effects of Ludwigia octovalvis (Jacq.) P. H. Raven (Onagraceae), an extract of which is widely consumed as a healthful drink in a number of countries. Using the fruit fly, Drosophila melanogaster, as a model organism, we demonstrated that L. octovalvis extract (LOE) significantly extended fly lifespan on a high, but not a low, calorie diet, indicating that LOE may regulate lifespan through a dietary restriction (DR)-related pathway. LOE also attenuated age-related cognitive decline in both flies and in the senescence-accelerated-prone 8 (SAMP8) mouse, without causing any discernable negative trade-offs, including water intake, food intake, fecundity, or spontaneous motor activity. LOE contained high levels of polyphenols and flavonoids, which possess strong DPPH radical scavenging activity, and was shown to attenuate paraquat-induced oxidative damage and lethality in flies. Gas chromatography-mass spectrometry (GC-MS) analyses identified 17 known molecules, of which β-sitosterol and squalene were the two most abundant. We further demonstrated that β-sitosterol was capable of extending lifespan, likely through activating AMP-activated protein kinase (AMPK) in the fat body of adult flies. Taken together, our data suggest that LOE is a potent anti-aging intervention with potential for treating age-related disorders.
Project description:Soil seed banks play a critical role in the maintenance of wetland plant communities and contribute to revegetation following disturbances. Analysis of the seed bank can therefore inform restoration planning and management. Emergence from seed banks may vary in response to hydrologic conditions and sediment disturbances. To assess the community-level impact of exotic Ludwigia hexapetala on soil seed banks, we compared differences in species composition of standing vegetation among invaded and non-invaded wetlands and the degree of similarity between vegetation and soil seed banks in northern California. To determine potential seed bank recruitment of L. hexapetala and associated plant species, we conducted a seedling emergence assay in response to inundation regime (drawdown vs. flooded) and sediment depth (surface vs. buried). Plant species richness, evenness, and Shannon's H' diversity were substantially lower in standing vegetation at L. hexapetala invaded sites as compared to non-invaded sites. Over 12 months, 69 plant taxa germinated from the seed banks, including L. hexapetala and several other exotic taxa. Seedling density varied among sites, being the highest (10,500 seedlings m-2) in surface sediments from non-invaded sites subjected to drawdown treatments. These results signal the need for invasive plant management strategies to deplete undesirable seed banks for restoration success.