Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-seq of Runx3 in SFZ chondrocytes.

ABSTRACT: ChIP-seq of Runx3 in SFZ chondrocytes.

PROVIDER: PRJNA660957 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12569435_1.fastq.gz	Fastqsanger.gz
	SRR12569435_2.fastq.gz	Fastqsanger.gz
	SRR12569436_1.fastq.gz	Fastqsanger.gz
	SRR12569436_2.fastq.gz	Fastqsanger.gz
	SRR12569437_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

ChIP-seq of Runx3 in SFZ chondrocytes.

Project description:Purpose: To demonstrate Runx3's association with organizing the extracellular matrix in SFZ chondrocytes. Method: Fragment DNA samples were collected by using Anti-FLAG or IgG, from SFZ chondrocytes treated with lipofection of RUNX3-FLAG Results: 21,730 raw peaks met the peak calling criterion. Conclusions: By GO analysis, the extracellular structure organization and collagen fibril organization terms were the most significantly enriched.

2020-09-03 | GSE157328 | GEO

RNA-seq of Runx3 cKO SFZ/DZ chondrocytes.

Project description:Purpose: To demonstrate Runx3's association with organizing the extracellular matrix in SFZ/DZ chondrocytes. Method: RNA samples were collected from SFZ/DZ chondrocytes of 5-day-old Col2a1-Cre;Runx3fl/fl and Runx3fl/fl mice. Results: 18 genes were up- or down-regulated by more than 2-fold in the Runx3 knockout in SFZ. Conclusions: However, genes organizing the extracellular matrix were down-regulated in Runx3 cKO DZ chondrocytes.

2020-09-04 | GSE157433 | GEO

RNA-seq of Runx3 cKO SFZ/DZ chondrocytes.

Project description:RNA-seq of Runx3 cKO SFZ/DZ chondrocytes.

| PRJNA661298 | ENA

Expression data from SFZ cells and costal chondrocytes

Project description:To understand difference between SFZ cells and costal chondrocytes, total RNAs from SFZ cells and costal chondrocytes were analyzed by microarray.

2023-09-06 | GSE242028 | GEO

Expression data from SFZ cells and costal chondrocytes

Project description:Expression data from SFZ cells and costal chondrocytes

| PRJNA1011239 | ENA

mRNA-seq analysis of superficial zone (SFZ) and deeper layers (deep AC) of articular cartilage of adult rats

Project description:We compared gene expression profiles of SFZ and deep AC of articular cartilage through laser microdissection (LMD) using adhesive tape, linear amplification of mRNA, and mRNA-seq analysis. gene expression profiles of SFZ and deep AC obtained from the proximal tibia of two adult rats

2014-05-08 | E-GEOD-57377 | biostudies-arrayexpress

ChIP-seq of Runx2-FLAG in primary chondrocytes.

Project description:ChIP-seq of Runx2-FLAG in primary chondrocytes.

| PRJNA693553 | ENA

Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha

Project description:Estrogen Receptor alpha (ERα) is a key driver of most breast cancers, and it is the target of endocrine therapies used in the clinic to treat women with ERα positive (ER+) breast cancer. The two methods ChIP-seq (chromatin immunoprecipitation coupled with deep sequencing) and RIME (Rapid Immunoprecipitation of Endogenous Proteins) have greatly improved our understanding of ERα function during breast cancer progression and in response to anti-estrogens. A critical component of both ChIP-seq and RIME protocols is the antibody that is used to pull down the bait protein. To date, most of the ChIP-seq and RIME experiments for the study of ERα have been performed using the sc-543 antibody from Santa Cruz Biotechnology. However, this antibody has been discontinued, thereby severely impacting the study of ERα in normal physiology as well as diseases such as breast cancer and ovarian cancer. Here, we compare the sc-543 antibody with other commercially available antibodies, and we show that 06-935 (EMD Millipore) and ab3575 (Abcam) antibodies can successfully replace the sc-543 antibody for ChIP-seq and RIME experiments.

2019-03-21 | PXD012930 | Pride

mRNA-seq analysis of superficial zone (SFZ) cells articular cartilage of 5-day-old mice

Project description:We compared gene expression profiles of SFZ cells of WT mice and Prg4KO mice using mRNA-seq analysis.

2021-04-20 | GSE148898 | GEO

Genome-wide maps of Runx3 bound regions in CD4 splenic dendritic cells.

Project description:ChIP-seq was conducted using FACS-isolated CD11chiMHCII+CD4+ splenic WT DC and anti-Runx3 antibodies (Ab). Two biological Runx3 IP repeats and two input controls from sorted CD4 DC

2014-06-22 | E-GEOD-48588 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data