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Caenorhabditis elegans

ABSTRACT: ChIP-seq on embryos (H3K27me3) and germline nuclei (H3K4me3) from C. elegans

PROVIDER: PRJNA661475 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12608190.fastq.gz	Fastqsanger.gz
	SRR12608190_1.fastq.gz	Fastqsanger.gz
	SRR12608190_2.fastq.gz	Fastqsanger.gz
	SRR12608191.fastq.gz	Fastqsanger.gz
	SRR12608191_1.fastq.gz	Fastqsanger.gz

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Project description:Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Transcription factors (TFs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire TF repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed TFs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 TFs with discrete autonomous germline functions. By identifying TFs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production.

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