Project description:Here we use DamID to identify Esg binding sites in Drosophila testes in order to investigate how it maintains somatic cyst stem cells.
Project description:To study the antagonism between Esg and Sc, we compared their direct binding sites identified by 3HA-esg and 3HA-sc ChIP-Seq. Through ChIP-Seq analysis, 14347 binding sites of Sc and 2765 binding sites of Esg were identified through p-value cutoff 1.00e-05. Among Sc binding sites, 10675 were protein-coding genes and 2678 localized to promoter region. For Esg, 2036 were protein-coding genes and 846 localized to promoter region. Through comparison, 2153 common binding sites were identified, and among these, 1620 were protein-coding genes and 659 localized to promoter region. A common binding region at the promoter of pros was identified.
Project description:Tissue stem cells divide asymmetrically to self-renew and generate differentiated progeny to maintain tissue homeostasis. Escargot (Esg) is a transcriptional repressor that is expressed in multiple stem cell populations in Drosophila melanogaster. Reduced esg function in intestinal stem cells (ISCs) causes a loss of ISCs and a biased differentiation of the daughter enteroblasts (EBs) toward the enteroendocrine (EE) cell fate. Loss of esg leads to an upregulation of differentiation factors and reduced Notch activity within EBs, indicating that Esg is a pivotal regulator of homeostasis in the intestine, supporting self-renewal to maintain a healthy stem cell pool as well as differentiation of progenitor cells. To identify potential transcriptional targets that mediate these phenotypes, in vivo DamID was used to generate the data deposited herein. 3 biological relicates were performed for Esg (with one dye-swap).
Project description:We carried out RBM46 eCLIP-seq (enhanced cross-linking immunoprecipitation coupled with sequencing) in wild-type testes and reported genome-wide occupancy of RBM46 in mouse testes.
Project description:Chromatin immunopurification with anti-CTCF was used to identify in vivo binding sites for CTCF in approximately 3% of the Drosophila genome using amplicon-based tiling arrays. This includes the Bithorax-complex, a known site of CTCF activity, the Antp-Complex, the Achaete scute-Complex and the Adh region. Reproducible binding identified new CTCF sites in the Adh region and the Bx-C. Keywords: ChIP-chip
Project description:Purpose: Genome-wide DNA-binding analysis for Stat92E and escargot(esg) in Drosophila testis cyst cells by DNA adenine methyltransferase identification(DamID).Methods: DNA adenine methyltransferase identification (DamID) on Stat92E and escargot(esg) driven by c587Gal4ts
Project description:Tissue stem cells divide asymmetrically to self-renew and generate differentiated progeny to maintain tissue homeostasis. Escargot (Esg) is a transcriptional repressor that is expressed in multiple stem cell populations in Drosophila melanogaster. Reduced esg function in intestinal stem cells (ISCs) causes a loss of ISCs and a biased differentiation of the daughter enteroblasts (EBs) toward the enteroendocrine (EE) cell fate. Loss of esg leads to an upregulation of differentiation factors and reduced Notch activity within EBs, indicating that Esg is a pivotal regulator of homeostasis in the intestine, supporting self-renewal to maintain a healthy stem cell pool as well as differentiation of progenitor cells. To identify potential transcriptional targets that mediate these phenotypes, in vivo DamID was used to generate the data deposited herein.